PERK antibody (pThr980)
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- Target See all PERK (EIF2AK3) Antibodies
- PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))
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Binding Specificity
- pThr980
- Reactivity
- Human, Mouse, Rat, Dog, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PERK antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Dog, Human, Mouse, Pig, Rat
- Predicted Reactivity
- Cow,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980
- Isotype
- IgG
- Top Product
- Discover our top product EIF2AK3 Primary Antibody
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells." in: Nutrients, Vol. 10, Issue 10, (2019) (PubMed).
: "Protective effects of Astragaloside IV on endoplasmic reticulum stress-induced renal tubular epithelial cells apoptosis in type 2 diabetic nephropathy rats." in: Biomedicine & pharmacotherapy, Vol. 109, pp. 84-92, (2019) (PubMed).
: "Functional Characterization of a Novel Truncating Mutation in Lamin A/C Gene in a Family with a Severe Cardiomyopathy with Conduction Defects." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 4, pp. 1559-1577, (2018) (PubMed).
: "Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma." in: OncoTargets and therapy, Vol. 11, pp. 5647-5655, (2018) (PubMed).
: "Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress." in: Aging, Vol. 10, Issue 10, pp. 2954-2972, (2018) (PubMed).
: "Tetramethylpyrazine suppresses angiotensin II-induced soluble epoxide hydrolase expression in coronary endothelium via anti-ER stress mechanism." in: Toxicology and applied pharmacology, Vol. 336, pp. 84-93, (2017) (PubMed).
: "Calcineurin β protects brain after injury by activating the unfolded protein response." in: Neurobiology of disease, Vol. 94, pp. 139-56, (2016) (PubMed).
: "The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca(2+) handling." in: Journal of cellular and molecular medicine, Vol. 20, Issue 11, pp. 2194-2207, (2016) (PubMed).
: "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 1, pp. 123-36, (2016) (PubMed).
: "Atg7 cooperates with Pten loss to drive prostate cancer tumor growth." in: Genes & development, Vol. 30, Issue 4, pp. 399-407, (2016) (PubMed).
: "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release." in: Chemical research in toxicology, Vol. 28, Issue 6, pp. 1326-37, (2015) (PubMed).
: "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." in: Molecular and cellular biochemistry, Vol. 389, Issue 1-2, pp. 151-8, (2014) (PubMed).
: "Neutral sphingomyelinase inhibition decreases ER stress-mediated apoptosis and inducible nitric oxide synthase in retinal pigment epithelial cells." in: Free radical biology & medicine, Vol. 72, pp. 113-23, (2014) (PubMed).
: "Inhibition of neutral sphingomyelinase decreases elevated levels of inducible nitric oxide synthase and apoptotic cell death in ocular hypertensive rats." in: Toxicology and applied pharmacology, (2014) (PubMed).
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Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells." in: Nutrients, Vol. 10, Issue 10, (2019) (PubMed).
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- Target
- PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))
- Alternative Name
- PERK (EIF2AK3 Products)
- Synonyms
- EIF2AK3 antibody, AI427929 antibody, Pek antibody, Perk antibody, PEK antibody, PERK antibody, WRS antibody, eukaryotic translation initiation factor 2 alpha kinase 3 antibody, EIF2AK3 antibody, Eif2ak3 antibody
- Background
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Synonyms: Pek, Perk, AI427929, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, Eif2ak3
Background: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
- Gene ID
- 13666
- UniProt
- Q9Z2B5
- Pathways
- Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response
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