PEA15 antibody (pSer116)

Catalog No. ABIN744698

Product Details anti-PEA15 Antibody

Target: PEA15 (phosphoprotein Enriched in Astrocytes 15 (PEA15))

Binding Specificity: pSer116

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEA15 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PEA15 around the phosphorylation site of Ser116

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PEA15 antibody (ABIN744698)

Kim, Kang: "PKC, AKT and ERK1/2-Mediated Modulations of PARP1, NF-κB and PEA15 Activities Distinctly Regulate Regional Specific Astroglial Responses Following Status Epilepticus." in: Frontiers in molecular neuroscience, Vol. 12, pp. 180, (2019) (PubMed).

Target Details for PEA15

Target: PEA15 (phosphoprotein Enriched in Astrocytes 15 (PEA15))

Alternative Name: PEA15 (PEA15 Products)

Synonyms: HMAT1 antibody, HUMMAT1H antibody, MAT1 antibody, MAT1H antibody, PEA-15 antibody, PED antibody, Pea15a antibody, PEA15 antibody, DKFZp459O1410 antibody, proliferation and apoptosis adaptor protein 15 antibody, phosphoprotein enriched in astrocytes 15 antibody, proliferation and apoptosis adaptor protein 15 L homeolog antibody, Astrocytic phosphoprotein PEA-15 antibody, PEA15 antibody, Pea15 antibody, pea15.L antibody, pea15 antibody

Background:

Synonyms: Astrocytic phosphoprotein PEA 15, Astrocytic phosphoprotein PEA15, HMAT 1, HMAT1, Homolog of mouse MAT 1 oncogene, Homolog of mouse MAT1 oncogene, HUMMAT 1H, HUMMAT1H, MAT 1, MAT 1H, MAT1, MAT1H, PEA 15, PEA-15_ PEA15 protein, PED, Phosphoprotein enriched in astrocytes 15, Phosphoprotein enriched in astrocytes 15kD, Phosphoprotein enriched in diabetes, PEA15_HUMAN.

Background: PED/PEA 15 (Phosphoprotein Enriched in Diabetes/Phosphoprotein Enriched in Astrocytes 15 kDa) is a widely expressed 15 kDa protein comprised of an N terminal region containing a canonical Death Effector Domain (DED) sequence and a nuclear export signal, and a C terminal region containing two serine phosphorylation sites. PED/PEA 15 has been implicated in the regulation of multiple cellular processes including apoptosis, integrin activation, and insulin sensitive glucose transport in insulin responsive cells. Phosphorylation of both serine 104 (a Protein Kinase C site) and serine 116 (a substrate of CaMKII and Akt) is required for PED/PEA 15 function.

Gene ID: 8682