NEDD4-2 antibody (pSer342)

Catalog No. ABIN744248

Product Details anti-NEDD4-2 Antibody

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Binding Specificity: pSer342

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4-2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NEDD4L around the phosphorylation site of Ser342

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Alternative Name: NEDD4L (NEDD4L Products)

Synonyms: NEDD4-2 antibody, NEDD4.2 antibody, RSP5 antibody, hNEDD4-2 antibody, nedd4 antibody, nedd4-2 antibody, NEDD4 antibody, Nedd4-2 antibody, 1300012C07Rik antibody, Nedd4b antibody, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase antibody, NEDD4L antibody, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase S homeolog antibody, neural precursor cell expressed, developmentally down-regulated gene 4-like antibody, NEDD4L antibody, LOC776799 antibody, nedd4l.S antibody, Nedd4l antibody

Background:

Synonyms: E3 ubiquitin protein ligase NEDD4 like protein, E3 ubiquitin-protein ligase NEDD4-like, E3 ubiquitin-protein ligase NEDD4-like protein, KIAA0439, NED4L_HUMAN, NEDD4 2, NEDD4 2c, NEDD4-2, NEDD4-2a, NEDD4.2, NEDD4b, NEDD4L, NEDD4La, NEDD4Lb, NEDD4Lc, NEDD4Ld, NEDD4Le, NEDD4Lf, NEDD4Lg, NEDD4Lh, NEDL3, Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase, Neural precursor cell expressed, developmentally down regulated 4 like, RSP5, Ubiquitin protein ligase Rsp5.

Background: E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.

Gene ID: 23327

Pathways: Negative Regulation of Transporter Activity