DUSP1 antibody (pSer359)

Catalog No. ABIN743933

Product Details anti-DUSP1 Antibody

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Binding Specificity: pSer359

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DUSP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MKP1 around the phosphorylation site of Ser359

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DUSP1

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Alternative Name: MKP1 (DUSP1 Products)

Background:

Synonyms: Mitogen activated protein kinase phosphatase 1, CL 100, CL100, Dual Specicity Phosphatase 1, Dual specicity protein phosphatase 1, Dual specicity protein phosphatase hVH1, DUSP 1, EC 3.1.3.16, EC 3.1.3.48, HVH 1, HVH1, MAP kinase phosphatase 1, MKP 1, MKP1, MKP-1, Protein tyrosine phosphatase CL100, PTPN 10, PTPN10, Serine/threonine specic protein phosphatase, VH 1, VH1, 3ch134, Mkp1, Ptpn16.

Background: The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues.

Gene ID: 1843