LRP6 antibody (pSer1490)

Catalog No. ABIN743558

Product Details anti-LRP6 Antibody

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Binding Specificity: pSer1490

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRP6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human LRP6 around the phosphorylation site of Ser1490

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-LRP6 antibody (ABIN743558)

Xiao, Fei, Hurley: "FGF2 crosstalk with Wnt signaling in mediating the anabolic action of PTH on bone formation." in: Bone reports, Vol. 9, pp. 136-144, (2018) (PubMed).

Boone, Arend, Johnston, Cooper, Gilchrist, Oelschlager, Grizzle, McGwin, Gangrade, Straughn, Buchsbaum: "Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 96, Issue 2, pp. 249-59, (2016) (PubMed).

Chen, Xu, Ye, Li, Gong, Zhang, Wu, Jia, Liu, Chen, Yang, Tang, Zhu, Ge, Zou: "Urotensin II inhibited the proliferation of cardiac side population cells in mice during pressure overload by JNK-LRP6 signalling." in: Journal of cellular and molecular medicine, Vol. 18, Issue 5, pp. 852-62, (2014) (PubMed).

Target Details for LRP6

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Alternative Name: LRP6 (LRP6 Products)

Synonyms: lrp6-A antibody, Xlrp6 antibody, adcad2 antibody, lrp6 antibody, LRP6 antibody, ADCAD2 antibody, C030016K15Rik antibody, Cd antibody, Gw antibody, LDL receptor related protein 6 antibody, LDL receptor related protein 6 L homeolog antibody, LDL receptor related protein 6 S homeolog antibody, low density lipoprotein receptor-related protein 6 antibody, low-density lipoprotein receptor-related protein 6 antibody, LRP6 antibody, lrp6.L antibody, lrp6.S antibody, lrp6 antibody, Lrp6 antibody, LOC590395 antibody

Background:

Synonyms: ADCAD2, Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6

Background: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).

Gene ID: 4040

UniProt: O75581

Pathways: WNT Signaling, Tube Formation