IRF3 antibody (pSer396)

Catalog No. ABIN742688

Product Details anti-IRF3 Antibody

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Binding Specificity: pSer396

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Monkey, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IRF3 antibody (ABIN742688)

Ando, Matsumoto, Taguchi, Kobayashi: "Poly (I:C) impairs NO donor-induced relaxation by overexposure to NO via the NF-kappa B/iNOS pathway in rat superior mesenteric arteries." in: Free radical biology & medicine, Vol. 112, pp. 553-566, (2017) (PubMed).

Le Bel, Gosselin: "Leukotriene B4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." in: PLoS ONE, Vol. 10, Issue 10, pp. e0139856, (2015) (PubMed).

Ichikawa, Sugiura, Koarai, Minakata, Kikuchi, Morishita, Oka, Kanai, Kawabata, Hiramatsu, Akamatsu, Hirano, Nakanishi, Matsunaga, Yamamoto, Ichinose: "TLR3 activation augments matrix metalloproteinase production through reactive nitrogen species generation in human lung fibroblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 11, pp. 4977-88, (2014) (PubMed).

Menasria, Boivin, Lebel, Piret, Gosselin, Boivin: "Both TRIF and IPS-1 Adaptor Proteins Contribute to the Cerebral Innate Immune Response against Herpes Simplex Virus 1 Infection." in: Journal of virology, Vol. 87, Issue 13, pp. 7301-8, (2013) (PubMed).

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3 (IRF3 Products)

Synonyms: irf3 antibody, IRF3 antibody, C920001K05Rik antibody, IRF-3 antibody, interferon regulatory factor 3 antibody, interferon regulatory factor 7 antibody, irf3 antibody, IRF3 antibody, irf-3 antibody, Irf3 antibody, IRF7 antibody

Background:

Synonyms: Interferon regulatory factor 3, IRF-3, IRF3

Background: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Gene ID: 3661

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades