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IRF3 antibody (pSer396)

Name: IRF3 antibody (pSer396)
SKU: ABIN742688
Price: 384.62 USD
Availability: InStock

IRF3 Reactivity: Human, Mouse, Rat, Monkey WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (IRF3 antibody (pSer396))

Formalin-fixed and paraffin embedded mouse stomach tissue labeled with Anti-Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (IRF3 antibody (pSer396))

IHC (p)

U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (IRF3 antibody (pSer396))

Formalin-fixed and paraffin embedded mouse stomach tissue labeled with Anti-Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (ABIN742688) at 1:200 followed by conjugation to the secondary antibody and DAB staining (IRF3 antibody (pSer396))

IHC

Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (ABIN742688) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (IRF3 antibody (pSer396))

5 images

(4 references)

Catalog No. ABIN742688

Datasheet as PDF

Target See all IRF3 Antibodies

IRF3 (Interferon Regulatory Factor 3 (IRF3))
Binding Specificity
15

14

14

14

14

9

8

7

6

5

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer396
Reactivity
142

89

53

30

18

3

2

2

2

1

1

1

1
Human, Mouse, Rat, Monkey
Host
150

15

2
Rabbit
Compatible Secondaries
Clonality
137

30
Polyclonal
Conjugate
88

12

9

8

4

4

4

4

4

4

2

2

2

2

2

2

2

2

2

2

2

2

2
This IRF3 antibody is un-conjugated
Application
121

69

35

32

28

26

18

10

9

9

5

4

3

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Monkey, Mouse, Rat

Predicted Reactivity

Dog,Cow,Sheep,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396

Isotype

IgG
anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-260) antibody
IRF3 Reactivity: Human WB, IHC, IF, ChIP Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-452) antibody
IRF3 Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3C8 unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-360) antibody
IRF3 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (AA 51-150) antibody
IRF3 Reactivity: Human, Mouse, Rat, Monkey, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (C-Term) antibody
IRF3 Reactivity: Human WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (pSer386) antibody
IRF3 Reactivity: Human, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (AA 1268-1341) antibody
IRF3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (AA 1-452) antibody
IRF3 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (C-Term) antibody
IRF3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Interferon Regulatory Factor 3 (IRF3) (pSer385) antibody
IRF3 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Ando, Matsumoto, Taguchi, Kobayashi: "Poly (I:C) impairs NO donor-induced relaxation by overexposure to NO via the NF-kappa B/iNOS pathway in rat superior mesenteric arteries." in: Free radical biology & medicine, Vol. 112, pp. 553-566, (2017) (PubMed).

Le Bel, Gosselin: "Leukotriene B4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." in: PLoS ONE, Vol. 10, Issue 10, pp. e0139856, (2015) (PubMed).

Ichikawa, Sugiura, Koarai, Minakata, Kikuchi, Morishita, Oka, Kanai, Kawabata, Hiramatsu, Akamatsu, Hirano, Nakanishi, Matsunaga, Yamamoto, Ichinose: "TLR3 activation augments matrix metalloproteinase production through reactive nitrogen species generation in human lung fibroblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 11, pp. 4977-88, (2014) (PubMed).

Menasria, Boivin, Lebel, Piret, Gosselin, Boivin: "Both TRIF and IPS-1 Adaptor Proteins Contribute to the Cerebral Innate Immune Response against Herpes Simplex Virus 1 Infection." in: Journal of virology, Vol. 87, Issue 13, pp. 7301-8, (2013) (PubMed).
Target

IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name

IRF3 (IRF3 Products)

Synonyms

irf3 antibody, IRF3 antibody, C920001K05Rik antibody, IRF-3 antibody, interferon regulatory factor 3 antibody, interferon regulatory factor 7 antibody, irf3 antibody, IRF3 antibody, irf-3 antibody, Irf3 antibody, IRF7 antibody

Background

Synonyms: Interferon regulatory factor 3, IRF-3, IRF3
Background: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Gene ID

3661

UniProt

Q14653

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades