IRAK1 antibody (pThr209)
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- Target See all IRAK1 Antibodies
- IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))
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Binding Specificity
- pThr209
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IRAK1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This phosphorylation site is homologous to Thr209 in Mouse and Rat.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr209 [RG(p-T)HN]
- Isotype
- IgG
- Top Product
- Discover our top product IRAK1 Primary Antibody
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- Application Notes
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WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))
- Alternative Name
- IRAK1 (IRAK1 Products)
- Synonyms
- IRAK1 antibody, IRAK antibody, pelle antibody, AA408924 antibody, IRAK-1 antibody, IRAK1-S antibody, Il1rak antibody, Plpk antibody, mPLK antibody, RGD1563841 antibody, interleukin 1 receptor associated kinase 1 antibody, interleukin-1 receptor-associated kinase 1 antibody, IRAK1 antibody, irak1 antibody, Irak1 antibody
- Background
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Synonyms: IRAK, pelle, Interleukin-1 receptor-associated kinase 1, IRAK-1, IRAK1
Background: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
- Gene ID
- 3654
- UniProt
- P51617
- Pathways
- NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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