CD163 antibody (AA 1001-1121)

Catalog No. ABIN741570

Product Details anti-CD163 Antibody

Target: CD163

Binding Specificity: AA 1001-1121

Reactivity: Human, Mouse, Rat, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Predicted Reactivity: Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD163/M130

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD163 antibody (ABIN741570)

Riuzzi, Beccafico, Sagheddu, Chiappalupi, Giambanco, Bereshchenko, Riccardi, Sorci, Donato: "Levels of S100B protein drive the reparative process in acute muscle injury and muscular dystrophy." in: Scientific reports, Vol. 7, Issue 1, pp. 12537, (2019) (PubMed).

Kobori, Hamasaki, Kitaura, Yamazaki, Nishinaka, Niwa, Nakao, Wake, Mori, Yoshino, Nishibori, Takahashi: "Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis." in: Frontiers in immunology, Vol. 9, pp. 334, (2019) (PubMed).

Yin, Chang, Xu: "Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration." in: BioMed research international, Vol. 2017, pp. 8428926, (2018) (PubMed).

Chen, Zhang, Wang, Chen, Yan, Zhang, Zhang: "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." in: American journal of translational research, Vol. 9, Issue 5, pp. 2352-2362, (2017) (PubMed).

Bobi, Solanes, Fernández-Jiménez, Galán-Arriola, Dantas, Fernández-Friera, Gálvez-Montón, Rigol-Monzó, Agüero, Ramírez, Roqué, Bayés-Genís, Sánchez-González, García-Álvarez, Sabaté, Roura, Ibáñez et al.: "Intracoronary Administration of Allogeneic Adipose Tissue-Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial ..." in: Journal of the American Heart Association, Vol. 6, Issue 5, (2017) (PubMed).

Gerzanich, Makar, Guda, Kwon, Stokum, Woo, Ivanova, Ivanov, Mehta, Morris, Bryan, Bever, Simard: "Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 177, (2017) (PubMed).

Lee, Liao, Wang, Yang, Luo, Varki, Qiu, Ren, Fu, Feng: "Preventive Inhibition of Liver Tumorigenesis by Systemic Activation of Innate Immune Functions." in: Cell reports, Vol. 21, Issue 7, pp. 1870-1882, (2017) (PubMed).

Dinulovic, Furrer, Di Fulvio, Ferry, Beer, Handschin: "PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle." in: Skeletal muscle, Vol. 6, pp. 38, (2017) (PubMed).

Tauchi, Tanaka, Kumamoto, Tokumoto, Sakimura, Sakurai, Kimura, Toyokawa, Amano, Kubo, Muguruma, Yashiro, Maeda, Ohira, Hirakawa: "Tumor-associated macrophages induce capillary morphogenesis of lymphatic endothelial cells derived from human gastric cancer." in: Cancer science, Vol. 107, Issue 8, pp. 1101-9, (2017) (PubMed).

Jacobsen, Mentzel, Olesen, Huby, Jørgensen, Barrès, Fredholm, Simar: "Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity." in: Journal of diabetes research, Vol. 2016, pp. 8539057, (2016) (PubMed).

Kugo, Zaima, Tanaka, Mouri, Yanagimoto, Hayamizu, Hashimoto, Sasaki, Sano, Yata, Urano, Setou, Unno, Moriyama: "Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm." in: Scientific reports, Vol. 6, pp. 31268, (2016) (PubMed).

Drel, Tan, Barnes, Gorin, Lee, Wagner: "Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 9, pp. 3026-38, (2016) (PubMed).

Das, Singh, Majid, Sherman, Mukhopadhyay, Wright, Martin, Dunn, Baker: "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." in: Advanced healthcare materials, Vol. 5, Issue 17, pp. 2248-60, (2016) (PubMed).

Chen, Shi, Zhang, Chen, Wang, Zhang, Tian, Yan, Li, Zhong, Xing, Zhang, Zhang: "Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing." in: Scientific reports, Vol. 5, pp. 18104, (2016) (PubMed).

Fernandez-Bustamante, Agazio, Wilson, Elkins, Domaleski, He, Baer, Moss, Wischmeyer, Repine et al.: "Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in ..." in: PLoS ONE, Vol. 10, Issue 7, pp. e0130764, (2016) (PubMed).

Tepeköylü, Lobenwein, Blunder, Kozaryn, Dietl, Ritschl, Pechriggl, Blumer, Bitsche, Schistek, Kotsch, Fritsch, Grimm, Holfeld: "Alteration of inflammatory response by shock wave therapy leads to reduced calcification of decellularized aortic xenografts in mice†." in: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, Vol. 47, Issue 3, pp. e80-90, (2015) (PubMed).

Ge, Li, Jiang, You, Liu, Zhong, Huang, Xing: "Integration of nondegradable polystyrene and degradable gelatin in a core-sheath nanofibrous patch for pelvic reconstruction." in: International journal of nanomedicine, Vol. 10, pp. 3193-201, (2015) (PubMed).

Franken, Klein, Spasova, Elsukova, Wiedwald, Welz, Knolle, Farle, Limmer, Kurts: "Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates." in: Scientific reports, Vol. 5, pp. 12940, (2015) (PubMed).

Target Details for CD163

Target: CD163

Alternative Name: Cd163/M130 (CD163 Products)

Synonyms: DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody

Background:

Synonyms: M13, MM13, Scavenger receptor cysteine-rich type 1 protein M13, Hemoglobin scavenger receptor, CD163, M130

Background: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH -dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Gene ID: 9332

UniProt: Q86VB7