CCL4 antibody (AA 26-92)

Catalog No. ABIN740805

Product Details anti-CCL4 Antibody

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Binding Specificity: AA 26-92

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rabbit

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CCL4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CCL4 antibody (ABIN740805)

Lee, Perry, Heywood, Reese, Bonner, Hatfield, Silver, Visconti, Hoffman, Tourkina: "Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis." in: Frontiers in pharmacology, Vol. 5, pp. 140, (2014) (PubMed).

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4 (CCL4 Products)

Synonyms: ACT2 antibody, AT744.1 antibody, G-26 antibody, HC21 antibody, LAG-1 antibody, LAG1 antibody, MIP-1-beta antibody, MIP1B antibody, MIP1B1 antibody, SCYA2 antibody, SCYA4 antibody, CCL4 antibody, K203 antibody, Mip1-b antibody, Scya4 antibody, MIP-1beta antibody, MIP-1BETA antibody, MIP1b antibody, SCY4A antibody, Act-2 antibody, MIP-1B antibody, Mip1b antibody, ACT-2 antibody, AT744.2 antibody, CCL4L antibody, SCYA4L antibody, SCYA4L1 antibody, SCYA4L2 antibody, C-C motif chemokine ligand 4 antibody, chemokine (C-C motif) ligand 4 antibody, chemokine (C-C motif) ligand 4-like 1 antibody, C-C motif chemokine ligand 4 like 1 antibody, CCL4 antibody, Ccl4 antibody, CCL4L1 antibody

Background:

Synonyms: MIP-1 Beta, Macrophage Inflammatory Protein 1 beta, MIP-1 Beta, MIP-1_, Secreted protein G 26, ACT 2, ACT2, AT744.1, CC chemokine ligand 4, CCL4, ccl4l 1, CCL4L1, Chemokine CC Mot Ligand 4, G 26 T lymphocyte secreted protein, HC21, LAG 1, LAG1, Lymphocyte activation gene 1, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1 beta, MGC104418, MGC126025, MGC126026, MIP1 beta, MIP1B, MIP1B1, PAT 744, Protein H400, SCYA2, SCYA4, Secreted protein G26, SIS gamma, Small inducible cytokine A4, T cell activation protein 2, CCL4_MOUSE,

Background: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.

Gene ID: 6351