Histone 3 antibody (AA 71-136)

Catalog No. ABIN739109

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: AA 71-136

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Rabbit,Drosophila

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Histone H3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Histone 3 antibody (ABIN739109)

Si, Meng, Gao, Hui, Li, Liu, Yang: "Linagliptin protects rat carotid artery from balloon injury and activates the NRF2 antioxidant pathway." in: Experimental animals, Vol. 68, Issue 1, pp. 81-90, (2019) (PubMed).

Zhang, Zhang, Li, Wang, Gu, Feng, Wei, Peng, Zhang, Su, Wang, Li, Yang, Wong, Yang, Ma: "Uncoupling Protein 2 Inhibits Myointimal Hyperplasia in Preclinical Animal Models of Vascular Injury." in: Journal of the American Heart Association, Vol. 6, Issue 10, (2018) (PubMed).

Joy, Gau, Castellucci, Prywes, Roy: "The myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms." in: The Journal of biological chemistry, Vol. 292, Issue 28, pp. 11777-11791, (2017) (PubMed).

Yang, Liu, Jiang, Wang, Zhang: "Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model." in: Frontiers in pharmacology, Vol. 8, pp. 44, (2017) (PubMed).

Shen, Wu, Liu, Zhao, Zhao: "Epigallocatechin-3-gallate alleviates paraquat-induced acute lung injury and inhibits upregulation of toll-like receptors." in: Life sciences, Vol. 170, pp. 25-32, (2016) (PubMed).

Qian, Zhang, Hu, Xu, Chen, Hu, Zhao, Liu: "Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status." in: Biomaterials, Vol. 70, pp. 12-22, (2015) (PubMed).

Duan, Liu, Liang, Yang, Xia, Wang, Chen, Yu: "Oestrogen receptor-mediated expression of Olfactomedin 4 regulates the progression of endometrial adenocarcinoma." in: Journal of cellular and molecular medicine, Vol. 18, Issue 5, pp. 863-74, (2014) (PubMed).

Tang, Chen, Liang, Rong, Yao, Yang, Wu: "Selective antegrade cerebral perfusion attenuating the TLR4/NF-?B pathway during deep hypothermia circulatory arrest in a pig model." in: Cardiology, Vol. 128, Issue 3, pp. 243-50, (2014) (PubMed).

Wang, Ma, Su: "NF-?B pathway contributes to cadmium-induced apoptosis of porcine granulosa cells." in: Biological trace element research, Vol. 153, Issue 1-3, pp. 403-10, (2013) (PubMed).

Liu, Ji, Zhang, Tong, Pan, Liu, Zhang, Huang, Su, Willard, Zheng: "Nonenzymatic glycation of high-density lipoprotein impairs its anti-inflammatory effects in innate immunity." in: Diabetes/metabolism research and reviews, Vol. 28, Issue 2, pp. 186-95, (2012) (PubMed).

Zhou, Zhou, Wang, Mu, Wu, Guo, Zhang, Wu: "Epigallocatechin-3-gallate inhibits proliferation and migration of human colon cancer SW620 cells in vitro." in: Acta pharmacologica Sinica, Vol. 33, Issue 1, pp. 120-6, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background:

Synonyms: Histone H3, H3.1, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, H3.2, HIST2H3A, HIST2H3C, HIST2H3D, H3F2, H3FM, H3.3, H3F3A, H3F3B, H3.3A, H3.3B, H3F3, PP781, H3.3C, H3F3C, H3.5, H3.1t, HIST3H3, H3FT

Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID: 3020

UniProt: P84243