N-Cadherin antibody (AA 701-800)

Catalog No. ABIN738726

Product Details anti-N-Cadherin Antibody

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Binding Specificity: AA 701-800

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Cow, Human, Mouse, Rat, Zebrafish (Danio rerio)

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human N-cadherin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-N-Cadherin antibody (ABIN738726)

Yang, Li, Qi, Li, Zhou, Qing, Zhang, Gao: "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." in: PeerJ, Vol. 5, pp. e3950, (2017) (PubMed).

Park, Kim, Lee: "Interaction-tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation." in: Journal of biomedical materials research. Part A, Vol. 105, Issue 1, pp. 42-50, (2016) (PubMed).

Liu, Cui, Ao, Zhou, Zhou, Yuan, Xiang, Liu, Cao et al.: "Aberrant methylation accounts for cell adhesion-related gene silencing during 3-methylcholanthrene and diethylnitrosamine induced multistep rat lung carcinogenesis associated with overexpression of ..." in: Toxicology and applied pharmacology, Vol. 251, Issue 1, pp. 70-8, (2011) (PubMed).

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: N-cadherin (CDH2 Products)

Synonyms: CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody

Background:

Synonyms: CDHN, NCAD, CD325, CDw325, Cadherin-2, Neural cadherin, N-cadherin, CDH2, CADH2_HUMAN

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

Gene ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane