LGR5 antibody (AA 810-907)

Catalog No. ABIN737916

Product Details anti-LGR5 Antibody

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Binding Specificity: AA 810-907

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGR5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse GPR49

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-LGR5 antibody (ABIN737916)

Su, Li, Liu, Ren, Tian, Liu, Qiu: "Ionizing radiation promotes advanced malignant traits in nasopharyngeal carcinoma via activation of epithelial-mesenchymal transition and the cancer stem cell phenotype." in: Oncology reports, Vol. 36, Issue 1, pp. 72-8, (2016) (PubMed).

Target Details for LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Alternative Name: GPR49/LGR5 (LGR5 Products)

Synonyms: lgr5-a antibody, lgr5a antibody, FEX antibody, Gpr49 antibody, GPR49 antibody, GPR67 antibody, GRP49 antibody, HG38 antibody, leucine-rich repeat containing G protein-coupled receptor 5 L homeolog antibody, leucine rich repeat containing G protein coupled receptor 5 antibody, leucine rich repeat containing G protein-coupled receptor 5 antibody, lgr5.L antibody, Lgr5 antibody, LGR5 antibody

Background:

Synonyms: FEX, Gpr49, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, Orphan G-protein coupled receptor FEX, Lgr5

Background: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Gene ID: 14160

UniProt: Q9Z1P4

Pathways: WNT Signaling