SP7 antibody (AA 331-431)

Catalog No. ABIN737811

Product Details anti-SP7 Antibody

Target: SP7 (Sp7 Transcription Factor (SP7))

Binding Specificity: AA 331-431

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rabbit

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Osterix

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SP7 antibody (ABIN737811)

Li, Xu, Xing, Qian, Chen, Gu, Guo, Lai, Zhao, Li, Wang, Wang, Deng: "A Conditional Knockout Mouse Model Reveals a Critical Role of PKD1 in Osteoblast Differentiation and Bone Development." in: Scientific reports, Vol. 7, pp. 40505, (2017) (PubMed).

Qian, Yan, Bai, Chen, Zhang, Yao, Xia: "Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 492-499, (2017) (PubMed).

Agarwal, Loder, Cholok, Peterson, Li, Fireman, Breuler, Hsieh, Ranganathan, Hwang, Drake, Li, Chan, Longaker, Levi: "Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury." in: Scientific reports, Vol. 6, pp. 32514, (2016) (PubMed).

Tang, Tan, Ni, Wang, Zhu, Fan, Chen, Yang, Zhang: "Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice." in: Materials science & engineering. C, Materials for biological applications, Vol. 70, Issue Pt 2, pp. 1000-1010, (2016) (PubMed).

Agarwal, Loder, Sorkin, Li, Shrestha, Zhao, Mishina, James, Levi: "Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification." in: Stem cells (Dayton, Ohio), Vol. 34, Issue 6, pp. 1692-701, (2016) (PubMed).

Wang, He, Liu, Liu, Zhou, Hao, Sun, Wang: "Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5514-5520, (2016) (PubMed).

Rajshankar, Wang, McCulloch: "Osteogenesis requires FAK-dependent collagen synthesis by fibroblasts and osteoblasts." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, Issue 3, pp. 937-953, (2016) (PubMed).

Ranganathan, Agarwal, Cholok, Loder, Li, Sung Hsieh, Wang, Buchman, Levi: "The role of the adaptive immune system in burn-induced heterotopic ossification and mesenchymal cell osteogenic differentiation." in: The Journal of surgical research, Vol. 206, Issue 1, pp. 53-61, (2016) (PubMed).

Kishi, Fujihara, Kawaguchi, Yamada, Nakayama, Yamamoto, Fujihara, Hamada, Satomura, Masutani: "PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway." in: International journal of molecular sciences, Vol. 16, Issue 10, pp. 24820-38, (2015) (PubMed).

Chen, Lazarenko, Zhang, Blackburn, Ronis, Badger: "Diet-derived phenolic acids regulate osteoblast and adipocyte lineage commitment and differentiation in young mice." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 29, Issue 5, pp. 1043-53, (2014) (PubMed).

Dong, Ge, Zhang, Fu, Zhang, Cheng, Jiang: "Phenotypic characterization of craniofacial bone marrow stromal cells: unique properties of enhanced osteogenesis, cell recruitment, autophagy, and apoptosis resistance." in: Cell and tissue research, Vol. 358, Issue 1, pp. 165-75, (2014) (PubMed).

Target Details for SP7

Target: SP7 (Sp7 Transcription Factor (SP7))

Alternative Name: Osterix (SP7 Products)

Background:

Synonyms: OSX, OI11, OI12, osterix, Transcription factor Sp7, Zinc finger protein osterix, SP7

Background: Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).

Gene ID: 121340

UniProt: Q8TDD2