SP7 antibody (AA 331-431)

Catalog No. ABIN737811

Product Details anti-SP7 Antibody

Target: SP7 (Sp7 Transcription Factor (SP7))

Binding Specificity: AA 331-431

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rabbit

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Osterix

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SP7 antibody (ABIN737811)

Li, Xu, Xing, Qian, Chen, Gu, Guo, Lai, Zhao, Li, Wang, Wang, Deng: "A Conditional Knockout Mouse Model Reveals a Critical Role of PKD1 in Osteoblast Differentiation and Bone Development." in: Scientific reports, Vol. 7, pp. 40505, (2017) (PubMed).

Qian, Yan, Bai, Chen, Zhang, Yao, Xia: "Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 492-499, (2017) (PubMed).

Agarwal, Loder, Cholok, Peterson, Li, Fireman, Breuler, Hsieh, Ranganathan, Hwang, Drake, Li, Chan, Longaker, Levi: "Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury." in: Scientific reports, Vol. 6, pp. 32514, (2016) (PubMed).

Tang, Tan, Ni, Wang, Zhu, Fan, Chen, Yang, Zhang: "Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice." in: Materials science & engineering. C, Materials for biological applications, Vol. 70, Issue Pt 2, pp. 1000-1010, (2016) (PubMed).

Agarwal, Loder, Sorkin, Li, Shrestha, Zhao, Mishina, James, Levi: "Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification." in: Stem cells (Dayton, Ohio), Vol. 34, Issue 6, pp. 1692-701, (2016) (PubMed).

Wang, He, Liu, Liu, Zhou, Hao, Sun, Wang: "Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5514-5520, (2016) (PubMed).

Rajshankar, Wang, McCulloch: "Osteogenesis requires FAK-dependent collagen synthesis by fibroblasts and osteoblasts." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, Issue 3, pp. 937-953, (2016) (PubMed).

Ranganathan, Agarwal, Cholok, Loder, Li, Sung Hsieh, Wang, Buchman, Levi: "The role of the adaptive immune system in burn-induced heterotopic ossification and mesenchymal cell osteogenic differentiation." in: The Journal of surgical research, Vol. 206, Issue 1, pp. 53-61, (2016) (PubMed).

Kishi, Fujihara, Kawaguchi, Yamada, Nakayama, Yamamoto, Fujihara, Hamada, Satomura, Masutani: "PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway." in: International journal of molecular sciences, Vol. 16, Issue 10, pp. 24820-38, (2015) (PubMed).

Chen, Lazarenko, Zhang, Blackburn, Ronis, Badger: "Diet-derived phenolic acids regulate osteoblast and adipocyte lineage commitment and differentiation in young mice." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 29, Issue 5, pp. 1043-53, (2014) (PubMed).

Dong, Ge, Zhang, Fu, Zhang, Cheng, Jiang: "Phenotypic characterization of craniofacial bone marrow stromal cells: unique properties of enhanced osteogenesis, cell recruitment, autophagy, and apoptosis resistance." in: Cell and tissue research, Vol. 358, Issue 1, pp. 165-75, (2014) (PubMed).

Target Details for SP7

Target: SP7 (Sp7 Transcription Factor (SP7))

Alternative Name: Osterix (SP7 Products)

Synonyms: BBBG1 antibody, FXY antibody, GBBB1 antibody, MIDIN antibody, OGS1 antibody, OS antibody, OSX antibody, RNF59 antibody, TRIM18 antibody, XPRF antibody, ZNFXY antibody, OI11 antibody, OI12 antibody, osterix antibody, Osx antibody, osx antibody, SP7 antibody, sp7 antibody, 6430578P22Rik antibody, C22 antibody, midline 1 antibody, Sp7 transcription factor antibody, uncharacterized LOC100063836 antibody, Sp7 transcription factor L homeolog antibody, Sp7 transcription factor 7 antibody, MID1 antibody, SP7 antibody, Sp7 antibody, sp7 antibody, LOC100063836 antibody, sp7.L antibody

Background:

Synonyms: OSX, OI11, OI12, osterix, Transcription factor Sp7, Zinc finger protein osterix, SP7

Background: Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).

Gene ID: 121340

UniProt: Q8TDD2