CCL2 antibody (AA 61-148)

Catalog No. ABIN737676

Product Details anti-CCL2 Antibody

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Binding Specificity: AA 61-148

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat MCP-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CCL2 antibody (ABIN737676)

Li, Fang, Tian, Wang, Ao, Hou, Tong, Fan, Bai: "Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4455-4462, (2018) (PubMed).

Kugo, Zaima, Onozato, Miyamoto, Hashimoto, Yanagimoto, Moriyama: "Suppressive effects of dietary EPA-rich fish oil on the degradation of elastin fibers in the aortic wall in nicotine-administered mice." in: Food & function, Vol. 8, Issue 8, pp. 2829-2835, (2017) (PubMed).

Kuracha, Thomas, Loggie, Govindarajan: "Patient-derived xenograft mouse models of pseudomyxoma peritonei recapitulate the human inflammatory tumor microenvironment." in: Cancer medicine, Vol. 5, Issue 4, pp. 711-9, (2016) (PubMed).

Jeong, Lee, Kim, Yang, Sung, Seong: "Irradiation-induced localization of IL-12-expressing mesenchymal stem cells to enhance the curative effect in murine metastatic hepatoma." in: International journal of cancer. Journal international du cancer, Vol. 137, Issue 3, pp. 721-30, (2015) (PubMed).

Guo, Feng, Yao, Sun, Zhu: "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." in: BMC nephrology, Vol. 15, pp. 110, (2014) (PubMed).

Iwasa, Arakaki, Honma, Ushio, Yamada, Kondo, Kurosawa, Kujiraoka, Tsunematsu, Kudo, Tanaka, Yoshimura, Harada, Hayashi, Ishimaru: "Aromatase controls Sjögren syndrome-like lesions through monocyte chemotactic protein-1 in target organ and adipose tissue-associated macrophages." in: The American journal of pathology, Vol. 185, Issue 1, pp. 151-61, (2014) (PubMed).

Su, Zhou, Wang, Yang, Li, Yu, Li: "The PPARβ/δ agonist GW501516 attenuates peritonitis in peritoneal fibrosis via inhibition of TAK1-NFκB pathway in rats." in: Inflammation, Vol. 37, Issue 3, pp. 729-37, (2014) (PubMed).

Guo, Zhang, Li, Xu: "Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats." in: Cardiovascular diabetology, Vol. 11, pp. 94, (2012) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1 (CCL2 Products)

Synonyms: GDCF-2 antibody, HC11 antibody, HSMCR30 antibody, MCAF antibody, MCP-1 antibody, MCP1 antibody, SCYA2 antibody, SMC-CF antibody, AI323594 antibody, JE antibody, Scya2 antibody, Sigje antibody, MCP-1A antibody, MCP1A antibody, C-C motif chemokine ligand 2 antibody, chemokine (C-C motif) ligand 2 antibody, C-C motif chemokine 2 antibody, CCL2 antibody, Ccl2 antibody, LOC101120093 antibody

Background:

Synonyms: MCP-1, Scya2, Sigje, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, Ccl2, Je, Mcp1

Background: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer, in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and targetcell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. Genetic variations in CCL2 determine Mycobacterium tuberculosis susceptibility Belongs to the intercrine beta (chemokine CC) family. The MCP-1( the monocyte chemotactic protein1) has many pits that regulates the function toward sex to turn to record the factor, it can induce to have relation with inflammation with the immunity various genes turn to record.The immunity MCP-1 set turns the male signal to mainly locate the afterbirth syrup inside.

Gene ID: 24770

UniProt: P14844

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection