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CAPN1 antibody (AA 625-713)

Name: CAPN1 antibody (AA 625-713)
SKU: ABIN737646
Price: 384.62 USD
Availability: InStock

CAPN1 Reactivity: Human, Mouse, Rat WB, IP, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse liver tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (CAPN1 antibody (AA 625-713))

IHC (p)

Antigen: 0. (CAPN1 antibody (AA 625-713))

ELISA

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (CAPN1 antibody (AA 625-713))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat lung tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (CAPN1 antibody (AA 625-713))

IHC (p)

4 images

(2 references)

Catalog No. ABIN737646

Datasheet as PDF

Target See all CAPN1 Antibodies

CAPN1 (Calpain 1 (CAPN1))
Binding Specificity
15

6

5

3

2

2

1

1

1

1

1

1

1
AA 625-713
Reactivity
49

36

36

5

2

2

2

2

1

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
55

6

1
Rabbit
Compatible Secondaries
Clonality
57

5
Polyclonal
Conjugate
22

8

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CAPN1 antibody is un-conjugated
Application
47

17

15

14

12

12

11

8

6

5

4

3

2

1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Cow,Sheep,Pig,Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse Calpain 1

Isotype

IgG

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CAPN1 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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CAPN1 Reactivity: Human WB, IP, ICC, IHC (p), IF Host: Rabbit Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 387-694) antibody
CAPN1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 75-343) antibody
CAPN1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Calpain 1 (CAPN1) antibody
CAPN1 Reactivity: Human, Mouse, Rat, Cow WB, IHC, IP, ELISA, IHC (p) Host: Mouse Monoclonal 15C10 unconjugated

anti-Calpain 1 (CAPN1) (AA 75-356) antibody
CAPN1 Reactivity: Rat WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 185-234) antibody
CAPN1 Reactivity: Human, Cow, Pig, Rabbit, Sheep, Goat, Monkey, Xenopus laevis, Zebrafish (Danio rerio), Bat, Chicken, Dog, Guinea Pig, Horse WB Host: Rabbit Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 300-349) antibody
CAPN1 Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Sheep, Goat WB Host: Rabbit Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 515-526) antibody
CAPN1 Reactivity: Human WB, IHC, ELISA, IHC (p) Host: Goat Polyclonal unconjugated

anti-Calpain 1 (CAPN1) (AA 185-356) antibody
CAPN1 Reactivity: Mouse WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Li, Chen, Lu, Zhang, Guan, Li, Wang: "Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer's Disease Mouse Model." in: International journal of molecular sciences, Vol. 18, Issue 8, (2017) (PubMed).

Miners, Renfrew, Swirski, Love: "Accumulation of ¿-synuclein in dementia with Lewy bodies is associated with decline in the ¿-synuclein-degrading enzymes kallikrein-6 and calpain-1." in: Acta neuropathologica communications, Vol. 2, Issue 1, pp. 164, (2014) (PubMed).
Target

CAPN1 (Calpain 1 (CAPN1))

Alternative Name

Calpain 1 (CAPN1 Products)

Synonyms

CAPN1 antibody, Capa-1 antibody, Capa1 antibody, mu-calpin antibody, Capn1 antibody, fj67g01 antibody, wu:fj67g01 antibody, calpain 1 antibody, zgc:55262 antibody, CAPN1 antibody, Capn1 antibody, zgc:55262 antibody

Background

Synonyms: Capa1, Capa-1, mu-calpin, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain, muCANP, Capn1, Canp1
Background: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Gene ID

12333

UniProt

O35350