NRF2 antibody (AA 430-480)

Catalog No. ABIN737271

Product Details anti-NRF2 Antibody

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Binding Specificity: AA 430-480

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Chicken, Human, Mouse, Pig, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nrf2

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NRF2 antibody (ABIN737271)

Xu, Zheng, Liu, Li, Yang, Shang: "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 6, pp. 2091-2104, (2018) (PubMed).

Zeng, Li, Zhang, Liu, Li, Xiong, He, Wang, Ye: "Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity." in: Transplantation proceedings, Vol. 49, Issue 2, pp. 366-372, (2017) (PubMed).

Zhang, Dong, Chen, Liu, Huang, Zhong, Cheng, Wang: "Nrf2 mediates the protective effects of homocysteine by increasing the levels of GSH content in HepG2 cells." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 597-602, (2017) (PubMed).

Yu, Bei, Li, Han, Zhong, Liu, Zhao, Zhu, Zhao: "In vitro the differences of inflammatory and oxidative reactions due to sulfur mustard induced acute pulmonary injury underlying intraperitoneal injection and intratracheal instillation in rats." in: International immunopharmacology, Vol. 47, pp. 78-87, (2017) (PubMed).

Shen, Wu, Wang, Zhao, Zhang, Li, Zhao: "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." in: International immunopharmacology, Vol. 46, pp. 16-22, (2017) (PubMed).

Zhang, Wang, Hao, Wang, Zhang, Muhammad, Qi, Li, Sun: "Nrf2 is crucial for the down-regulation of Cyp7a1 induced by arachidonic acid in Hepg2 cells." in: Environmental toxicology and pharmacology, Vol. 52, pp. 21-26, (2017) (PubMed).

Cao, Huang, Dou, Xiang, Dong: "Cyanidin suppresses autophagic activity regulating chondrocyte hypertrophic differentiation." in: Journal of cellular physiology, Vol. 233, Issue 3, pp. 2332-2342, (2017) (PubMed).

Kouam, Yuan, Njayou, He, Tsayem, Oladejo, Song, Moundipa, Gao: "Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity." in: Frontiers in pharmacology, Vol. 8, pp. 653, (2017) (PubMed).

Jiang, Li, He, Yao, Bian, Wang, Zhu: "Protective Effects of Asiatic Acid Against Spinal Cord Injury-Induced Acute Lung Injury in Rats." in: Inflammation, Vol. 39, Issue 6, pp. 1853-1861, (2016) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Wang, Peng, Wei, Wei, Wang, Ma, Yao, Fu, Zu: "Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzyme expression via PI3K/AKT and ERK1/2 pathway." in: Biochimica et biophysica acta, Vol. 1850, Issue 9, pp. 1751-61, (2015) (PubMed).

Yu, Shi, Zhao, Sun, Gao, Sun, Qi: "Triptolide Attenuates Myocardial Ischemia/Reperfusion Injuries in Rats by Inducing the Activation of Nrf2/HO-1 Defense Pathway." in: Cardiovascular toxicology, (2015) (PubMed).

Lv, Mao, Wu, Lu, Ding, Gu, Wu, Shi: "Neuroprotective effect of allicin in a rat model of acute spinal cord injury." in: Life sciences, Vol. 143, pp. 114-23, (2015) (PubMed).

Lee, Lee, Wu: "Actinidia callosa peel (kiwi fruit) ethanol extracts protected neural cells apoptosis induced by methylglyoxal through Nrf2 activation." in: Pharmaceutical biology, Vol. 52, Issue 5, pp. 628-36, (2014) (PubMed).

Wang, Fang, Huang, Chen, Fan, Wang: "The chronic effects of low lead level on the expressions of Nrf2 and Mrp1 of the testes in the rats." in: Environmental toxicology and pharmacology, Vol. 35, Issue 1, pp. 109-16, (2013) (PubMed).

Lee, Hsu, Huang, Chang, Hsu, Pan: "Effects of monascin on anti-inflammation mediated by Nrf2 activation in advanced glycation end product-treated THP-1 monocytes and methylglyoxal-treated wistar rats." in: Journal of agricultural and food chemistry, Vol. 61, Issue 6, pp. 1288-98, (2013) (PubMed).

Hsu, Lee, Li, Hsu, Pan: "Monascin and AITC attenuate methylglyoxal-induced PPAR? phosphorylation and degradation through inhibition of the oxidative stress/PKC pathway depending on Nrf2 activation." in: Journal of agricultural and food chemistry, Vol. 61, Issue 25, pp. 5996-6006, (2013) (PubMed).

Lee, Hsu, Hsu, Pan: "Dimerumic acid attenuates receptor for advanced glycation endproducts signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promotes methylglyoxal metabolism into d-lactic acid." in: Free radical biology & medicine, Vol. 60, pp. 7-16, (2013) (PubMed).

Lee, Lee, Cheng, Chang, Hsu, Wu: "Graptopetalum paraguayense and resveratrol ameliorates carboxymethyllysine (CML)-induced pancreas dysfunction and hyperglycemia." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 62, pp. 492-8, (2013) (PubMed).

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: Nrf2 (NFE2L2 Products)

Synonyms: Nrf2 antibody, nfe2l2 antibody, wu:fc15g09 antibody, wu:fj67e03 antibody, NF-E2R2 antibody, NRF2 antibody, AI194320 antibody, nuclear factor, erythroid 2-like 2a antibody, nuclear factor, erythroid 2 like 2 S homeolog antibody, nuclear factor, erythroid 2 like 2 antibody, nuclear factor, erythroid derived 2, like 2 antibody, nuclear factor, erythroid 2-like 2 antibody, nfe2l2a antibody, nfe2l2.S antibody, NFE2L2 antibody, Nfe2l2 antibody

Background:

Synonyms: NRF2, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2

Background: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Gene ID: 4780

UniProt: Q16236

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling