Cytokeratin 5 antibody (AA 331-430)

Catalog No. ABIN737061

Product Details anti-Cytokeratin 5 Antibody

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Binding Specificity: AA 331-430

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CK5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5 (KRT5 Products)

Synonyms: 3300001P10Rik antibody, AW146334 antibody, CK5 antibody, K5 antibody, Krt2-5 antibody, Tfip8 antibody, DDD antibody, DDD1 antibody, EBS2 antibody, KRT5A antibody, Sak57 antibody, ckii antibody, wu:fb02h09 antibody, wu:fj02d02 antibody, wu:fk78b12 antibody, zfCKII antibody, KRT75 antibody, CK-5 antibody, Keratin-5 antibody, keratin 5 antibody, Krt5 antibody, KRT5 antibody, krt5 antibody

Background:

Synonyms: Cytokeratin 5, Keratin, type II cytoskeletal 5, CK-5, CK 5, CK5, Keratin-5, K5, 58 kDa cytokeratin, KRT5, DDD, EBS 2, EBS2, K5, Keratin 5 epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types, Keratin 5, Keratin Type II Cytoskeletal 5, Keratin5, KRT 5, KRT 5A, KRT5, Cytokeratin-5, Cytokeratin5, DDD, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K2C5_HUMAN, Keratin 5, Keratin, Keratin Type II Cytoskeletal 5, Keratin5, KRT5A, type II cytoskeletal 5, Type-II keratin Kb5.

Background: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.

Molecular Weight: 64kDa

Gene ID: 3852