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K-RAS antibody (AA 25-130)

Name: K-RAS antibody (AA 25-130)
SKU: ABIN736671
Price: 384.62 USD
Availability: InStock

KRAS Reactivity: Human, Rat, Mouse WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded: human endometrium tissue labeled with Anti-KRAS Polyclonal Antibody (ABIN736671), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (K-RAS antibody (AA 25-130))

IHC

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (K-RAS antibody (AA 25-130))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nuclei. (K-RAS antibody (AA 25-130))

IF (p)

3 images

Catalog No. ABIN736671

Datasheet as PDF

Target See all K-RAS (KRAS) Antibodies

K-RAS (KRAS) (GTPase Kras (KRAS))
Binding Specificity
15

11

10

8

6

4

2

2

1

1

1

1

1

1

1
AA 25-130
Reactivity
61

38

31

5

4

3

3

3

3

3

3

2

2

2

2

1

1

1
Human, Rat, Mouse
Host
64

10
Rabbit
Compatible Secondaries
Clonality
64

10
Polyclonal
Conjugate
29

8

4

4

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This K-RAS antibody is un-conjugated
Application
64

30

23

14

12

12

10

5

5

4

2

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human K-ras

Isotype

IgG

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KRAS Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3B10-2F2 unconjugated

anti-GTPase Kras (KRAS) (AA 16-125) antibody
KRAS Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4F3 unconjugated

anti-GTPase Kras (KRAS) (AA 146-174) antibody
KRAS Reactivity: Human, Rat, Mouse WB, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 2-168) antibody
KRAS Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (N-Term) antibody
KRAS Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Drosophila melanogaster, Pig, Bat, Chicken, Xenopus laevis, Hamster, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (pSer89) antibody
KRAS Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (N-Term) antibody
KRAS Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 1-189) antibody
KRAS Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (C-Term) antibody
KRAS Reactivity: Human, Rat, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-GTPase Kras (KRAS) (AA 1-188) antibody
KRAS Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1E4-1B10 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name

KRAS (KRAS Products)

Synonyms

wu:fa04e08 antibody, wu:fc14b12 antibody, wu:fc23g10 antibody, wu:fj89d12 antibody, zgc:85725 antibody, Kras2 antibody, c-Ki-ras antibody, p21 antibody, C-K-RAS antibody, CFC2 antibody, K-RAS2A antibody, K-RAS2B antibody, K-RAS4A antibody, K-RAS4B antibody, KI-RAS antibody, KRAS1 antibody, KRAS2 antibody, NS antibody, NS3 antibody, RASK2 antibody, AI929937 antibody, K-ras antibody, Ki-ras antibody, Kras-2 antibody, p21B antibody, ras antibody, k-ras antibody, kras antibody, kras-a antibody, kras-b antibody, rask2 antibody, K-RAS antibody, p21ras antibody, KRAS proto-oncogene, GTPase antibody, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog antibody, Kirsten rat sarcoma viral oncogene homolog antibody, semicolonial-7 antibody, Ras family protein antibody, GTPase KRas antibody, Kirsten rat sarcoma viral oncogene homolog S homeolog antibody, KRAS antibody, kras antibody, Kras antibody, smco-7 antibody, PGTG_03066 antibody, Tsp_02666 antibody, Tsp_01642 antibody, rask antibody, kras.S antibody

Target Type

Viral Protein

Background

Synonyms: NS, NS3, CFC2, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, GTPase KRas, K-Ras 2, c-Ki-ras, KRAS
Background: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838).

Gene ID

3845

UniProt

P01116