TLR4 antibody (AA 751-835)

Catalog No. ABIN736491

Product Details anti-TLR4 Antibody

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Binding Specificity: AA 751-835

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat TLR4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TLR4 antibody (ABIN736491)

Kulhankova, Kinney, Stach, Gourronc, Grumbach, Klingelhutz, Salgado-Pabón: "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." in: Infection and immunity, (2018) (PubMed).

Xu, Zheng, Liu, Li, Yang, Shang: "Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 6, pp. 2091-2104, (2018) (PubMed).

Harasymowicz, Clement, Azfer, Burnett, Salter, Simpson: "Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and Class III Obesity in Patients With Osteoarthritis." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 69, Issue 7, pp. 1396-1406, (2017) (PubMed).

Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, (2017) (PubMed).

Arfian, Muflikhah, Soeyono, Sari, Tranggono, Anggorowati, Romi: "Vitamin D Attenuates Kidney Fibrosis via Reducing Fibroblast Expansion, Inflammation, and Epithelial Cell Apoptosis." in: The Kobe journal of medical sciences, Vol. 62, Issue 2, pp. E38-44, (2016) (PubMed).

Kawakita, Fujimoto, Liu, Nakano, Nakatsuka, Suzuki: "Effects of Toll-Like Receptor 4 Antagonists Against Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage in Mice." in: Molecular neurobiology, (2016) (PubMed).

Wang, Shi, Lv, Xu, Li, Ge, Xiao, Geng, Liu: "Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-?B Activation in Microglial Cells." in: Inflammation, (2015) (PubMed).

Namisaki, Noguchi, Moriya, Kitade, Aihara, Douhara, Nishimura, Takeda, Okura, Kawaratani, Takaya, Seki, Yoshiji: "Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis." in: Journal of gastroenterology, (2015) (PubMed).

Yang, Wang, Zhang, Yang, Lu, Zhang, Li, Zhang, Li: "Astragaloside IV attenuates inflammatory cytokines by inhibiting TLR4/NF-?B signaling pathway in isoproterenol-induced myocardial hypertrophy." in: Journal of ethnopharmacology, Vol. 150, Issue 3, pp. 1062-70, (2014) (PubMed).

Chen, Yu, Fan, Bian, Ma, Lu, Jin: "Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-?B) and induces cytokine production via Toll-like receptors." in: Carbohydrate polymers, Vol. 105, pp. 113-20, (2014) (PubMed).

Xing, Zheng, Zhang, Wang, Xing: "Relationship between toll-like receptor 4 levels in aorta and severity of atherosclerosis." in: The Journal of international medical research, Vol. 42, Issue 4, pp. 958-965, (2014) (PubMed).

Takanashi, Tomosada, Villena, Murata, Takahashi, Chiba, Tohno, Shimazu, Aso, Suda, Ikegami, Itoh, Kawai, Saito, Alvarez, Kitazawa: "Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation." in: BMC microbiology, Vol. 13, pp. 54, (2013) (PubMed).

Tang, Zhou, Wang, Zhang, Wang, Li, Huang: "Expression of TRAF6 and pro-inflammatory cytokines through activation of TLR2, TLR4, NOD1, and NOD2 in human periodontal ligament fibroblasts." in: Archives of oral biology, Vol. 56, Issue 10, pp. 1064-72, (2011) (PubMed).

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4 (TLR4 Products)

Synonyms: ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody

Background:

Synonyms: Lps, Ly87, Ran/M1, Rasl2-8, Toll-like receptor 4, CD284, Tlr4

Background: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.

Gene ID: 21898

UniProt: Q9QX05

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins