PTTG1 antibody (AA 101-202)

Catalog No. ABIN735803

Product Details anti-PTTG1 Antibody

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Binding Specificity: AA 101-202

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTTG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PTTG

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Alternative Name: Securin/PTTG (PTTG1 Products)

Synonyms: EAP1 antibody, HPTTG antibody, PTTG antibody, TUTR1 antibody, PTTG1 antibody, securin antibody, AW555095 antibody, C87862 antibody, Pttg antibody, Pttg3 antibody, Securin antibody, Protein Cut2 antibody, pituitary tumor-transforming 1 antibody, pituitary tumor-transforming gene 1 antibody, securin, sister chromatid separation inhibitor antibody, PTTG1 antibody, Pttg1 antibody, cut2 antibody

Background:

Synonyms: AW555095, C87862, Cut2, EAP 1, EAP1, ESP1 ASSOCIATED PROTEIN 1, Esp1-associated protein, hPTTG, MGC126883, MGC138276, Pds1, Pituitary tumor transforming 1, Pituitary tumor transforming protein 1, Pituitary tumor-transforming 1, isoform CRA_a, Pituitary tumor-transforming 1, isoform CRA_b, Pituitary tumor-transforming gene 1, Pituitary tumor-transforming gene 1 protein, PTTG 1, PTTG, PTTG1, PTTG1 protein, Pttg3, TUMOR TRANSFORMING 1, Tumor transforming protein 1, TUMOR-TRANSFORMING 1, TUTR 1, TUTR1.

Background: Securin is an anaphase inhibitor and plays an important role in DNA damage and spindle check point pathways. It inhibits sister chromatid separation by binding and inhibiting Esp 1, a cystein protease that causes cleavage of the cohesin Scc 1 that binds the sister chromatids together. It is responsible for targeting of Esp 1 to the nucleus and its binding to the spindle. Degradation of Securin occurs shortly before anaphase, which liberates Esp 1 and is a pre-requisite for anaphase entry. It is targeted for degradation by ubiquitination mediated bycyclosome / anaphase promoting complex (APC)functioning as a ubiquitin ligase. In response to DNA damage Chk 1 phosphorylates Securin to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase. Securin is highly expressed in various human tumors, including pituitary, adrenal, kidney, liver, and ovarian tumors, and in cell lines derived from pituitary tumors, breast tumors, endometrial tumors, and ovarian tumors.

Gene ID: 9232