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ITGAL antibody (AA 251-350)

Name: ITGAL antibody (AA 251-350)
SKU: ABIN734678
Price: 384.62 USD
Availability: InStock

ITGAL Reactivity: Human, Rat, Rabbit FACS, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Human U937 cells probed with Integrin alpha L/CD11a Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (ITGAL antibody (AA 251-350))

FACS

Paraformaldehyde-fixed, paraffin embedded rat adrendal gland, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Integrin alpha L/CD11a Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (ITGAL antibody (AA 251-350))

IHC (p)

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti CD11a Polyclonal Antibody, Unconjugated (ABIN734678) at 1:200 followed by conjugation to the secondary antibody and DAB staining (ITGAL antibody (AA 251-350))

IHC

3 images

Catalog No. ABIN734678

Datasheet as PDF

Target See all ITGAL Antibodies

ITGAL (Integrin alpha-L (ITGAL))
Binding Specificity
8

8

7

6

5

5

2

2

2

1

1

1

1

1

1
AA 251-350
Reactivity
129

59

15

3

2

2

1

1

1

1

1
Human, Rat, Rabbit
Host
95

54

38

1
Rabbit
Compatible Secondaries
Clonality
141

46

1
Polyclonal
Conjugate
61

28

24

19

8

6

5

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This ITGAL antibody is un-conjugated
Application
131

45

35

32

32

24

23

16

8

5

5

5

2

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity

Human, Rabbit, Rat

Predicted Reactivity

Mouse,Dog,Cow,Sheep,Pig,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Integrin alpha L

Isotype

IgG

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ITGAL Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha-L (ITGAL) antibody
ITGAL Reactivity: Human FACS, IP, IHC Host: Mouse Monoclonal 38 unconjugated

anti-Integrin alpha-L (ITGAL) antibody
ITGAL Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha-L (ITGAL) antibody
ITGAL Reactivity: Mouse FACS, IP, IHC (fro) Host: Rat Monoclonal M17-4 unconjugated

anti-Integrin alpha-L (ITGAL) antibody
ITGAL Reactivity: Mouse FACS, IP, Func, IHC (fro) Host: Rat Monoclonal M17-4 unconjugated

anti-Integrin alpha-L (ITGAL) (AA 201-500) antibody
ITGAL Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha-L (ITGAL) antibody
ITGAL Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-25 unconjugated

anti-Integrin alpha-L (ITGAL) antibody
ITGAL Reactivity: Human FACS, IP, Func Host: Mouse Monoclonal MEM-25 unconjugated

anti-Integrin alpha-L (ITGAL) antibody (Biotin)
ITGAL Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-25 Biotin

Application Notes

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

ITGAL (Integrin alpha-L (ITGAL))

Alternative Name

Integrin alpha L/CD11a (ITGAL Products)

Synonyms

CD11A antibody, LFA-1 antibody, LFA1A antibody, (p180) antibody, Cd11a antibody, LFA-1A antibody, Ly-15 antibody, Ly-21 antibody, CD11a antibody, ITGAL antibody, integrin subunit alpha L antibody, integrin alpha L antibody, ITGAL antibody, Itgal antibody

Background

Synonyms: CD11A, LFA-1, LFA1A, Integrin alpha-L, CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, ITGAL
Background: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Gene ID

3683

UniProt

P20701

Pathways

Activated T Cell Proliferation, Integrin Complex