GATA5 antibody (AA 161-260)

Catalog No. ABIN734348

Product Details anti-GATA5 Antibody

Target: GATA5 (GATA Binding Protein 5 (GATA5))

Binding Specificity: AA 161-260

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GATA-5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GATA5

Target: GATA5 (GATA Binding Protein 5 (GATA5))

Alternative Name: GATA5 (GATA5 Products)

Synonyms: GATAS antibody, bB379O24.1 antibody, GATA-5 antibody, GATA5 antibody, xGATA5 antibody, xGATA-5 antibody, xGATA5a antibody, xGATA5b antibody, xGATA-5a antibody, xGATA-5b antibody, bb379o24.1 antibody, gata-4a antibody, gata-5a antibody, gata5 antibody, gata-5b antibody, cb634 antibody, fau/gata5 antibody, gta5 antibody, GATA binding protein 5 antibody, GATA binding protein 5 S homeolog antibody, GATA binding protein 5 L homeolog antibody, GATA5 antibody, Gata5 antibody, gata5 antibody, gata5.S antibody, gata5.L antibody

Background:

Synonyms: GATA binding protein 5, GATA-binding factor 5, GATA5, GATA-5, GATA5_HUMAN, Transcription factor GATA-5.

Background: Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

Gene ID: 140628