Elastin antibody (AA 681-786)

Catalog No. ABIN734003

Product Details anti-Elastin Antibody

Target: Elastin (ELN)

Binding Specificity: AA 681-786

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Elastin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #028751 (Immunohistochemistry)

by: Beth Israel Deaconess Medical Center Confocal Imaging Core

No.: #028751

Date: 09/25/2013

Lot Number: 120306

Method validated: Immunohistochemistry

Positive Control: Mouse heart tissue

Negative Control: Mouse adipose tissue

Notes: Signal was detected in positive control tissue and not detected in negative control tissue.

Validation Images

Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue stained with anti-Elastin antibody (brown). Panel 2: Staining of mouse adipose tissue with anti-Elastin antibody. Panel 3: Staining of mouse heart tissue with isotype control antibody. Panel 4: Staining of mouse heart tissue with secondary antibody only. All panels are counterstained with hematoxylin (blue).

Full Methods

Primary Antibody:

• Antibody: Elastin (ELN) (C-Term) antibody
• Catalog number: ABIN734003
• Lot number: 120306

Secondary Antibody:

• Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)

Full Protocol:

• Immunohistochemistry was performed manually.
• Sections were deparaffinized and rehydrated.
• Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
• Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
• Sections were washed x 3 in Tris buffered saline (TBS).
• Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
• Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
• Sections were washed x 3 in Tris buffered saline.
• Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
• Sections were washed x 3 in Tris buffered saline.
• Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
• Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
• Sections were washed x 3 in Distilled Water.
• Sections were counterstained with hematoxylin for 1 min.
• Sections were washed x 1 in Distilled Water.
• Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.

Experimental Notes: None

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Elastin antibody (ABIN734003)

Hasegawa, Sato, Shirai, Watanabe, Yamamoto, Watanabe, Nohtomi, Hirano, Watanabe: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." in: PLoS ONE, Vol. 9, Issue 12, pp. e110866, (2014) (PubMed).

Konii, Sato, Kikuchi, Okiyama, Watanabe, Hasegawa, Yamamoto, Itoh, Hirano, Watanabe: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." in: Hypertension, Vol. 62, Issue 5, pp. 942-50, (2013) (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).

Ikeda, Yada, Takase: "[Syntheses of 1,3,4-thiadiazole derivatives]." in: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, (1970) (PubMed).

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: Elastin (ELN Products)

Synonyms: SVAS antibody, WBS antibody, WS antibody, AI385707 antibody, AI480567 antibody, E030024M20Rik antibody, RATTREL11 antibody, TREL11 antibody, Trela antibody, Trela26 antibody, ELN antibody, elastin antibody, ELN antibody, Eln antibody, LOC574132 antibody, Tcur_2481 antibody, Kfla_2289 antibody, LOC100620140 antibody

Background:

Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN

Background: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Gene ID: 2006

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization