For best experience we recommend to activate Javascript in your browser.

ERCC1 antibody (AA 151-250)

Name: ERCC1 antibody (AA 151-250)
SKU: ABIN733553
Price: 384.62 USD
Availability: InStock

ERCC1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-ERCC1 Polyclonal Antibody, Unconjugated (ABIN733553) at 1:200 followed by conjugation to the secondary antibody and DAB staining (ERCC1 antibody (AA 151-250))

IHC

Paraformaldehyde-fixed, paraffin embedded rat testis tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-ERCC1 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining (ERCC1 antibody (AA 151-250))

IHC (p)

IF (p)

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-ERCC1 Polyclonal Antibody, Unconjugated (ABIN733553) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining (ERCC1 antibody (AA 151-250))

IHC

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-ERCC1 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining (ERCC1 antibody (AA 151-250))

IHC (p)

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-ERCC1 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (ERCC1 antibody (AA 151-250))

IHC (p)

6 images

Catalog No. ABIN733553

Datasheet as PDF

Target See all ERCC1 Antibodies

ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))
Binding Specificity
14

10

6

6

5

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 151-250
Reactivity
75

34

23

5

5

3

3

2

2

1

1

1

1

1
Human, Mouse, Rat
Host
47

26

2
Rabbit
Compatible Secondaries
Clonality
48

27
Polyclonal
Conjugate
52

4

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1
This ERCC1 antibody is un-conjugated
Application
65

37

15

12

12

12

12

7

7

3

3

2

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human ERCC1

Isotype

IgG
anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-297) antibody
ERCC1 Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 5A2D1 unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 151-297) antibody
ERCC1 Reactivity: Human ELISA, FACS, ICC Host: Mouse Monoclonal 1E5B3 unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 191-281) antibody
ERCC1 Reactivity: Human IHC, StM Host: Mouse Monoclonal ERCC1-2318 unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 268-297), (C-Term) antibody
ERCC1 Reactivity: Human WB Host: Mouse Monoclonal 752CT13-2-5 unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-120) antibody
ERCC1 Reactivity: Human ELISA, FACS Host: Mouse Monoclonal 3F9E9 unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-323) antibody
ERCC1 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (Internal Region) antibody
ERCC1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (Internal Region) antibody
ERCC1 Reactivity: Human, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 207-281) antibody
ERCC1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3A7 unconjugated

anti-Excision Repair Cross Complementing Polypeptide-1 (ERCC1) (AA 1-323) antibody
ERCC1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name

ERCC1 (ERCC1 Products)

Background

Synonyms: UV2, COFS4, RAD1, DNA excision repair protein ERCC-1, ERCC1
Background: Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

Gene ID

2067

UniProt

P07992

Pathways

DNA Damage Repair, Production of Molecular Mediator of Immune Response