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CD15 antibody (AA 251-295)

Name: CD15 antibody (AA 251-295)
SKU: ABIN733193
Price: 384.62 USD
Availability: InStock

FUT4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p), ELISA, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Human HL-60 cells probed with CD15 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (CD15 antibody (AA 251-295))

FACS

MCF-7 probed with Rabbit Anti-CD15/Fut4/SSEA-1 Polyclonal Antibody at 1:5000 for 90 min at 37˚C. (CD15 antibody (AA 251-295))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD15/Fut4/SSEA-1 Polyclonal Antibody, Unconjugated (ABIN733193) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD15 antibody (AA 251-295))

IHC

Lane 1: MCF-7 lysates probed with CD15/Fut4/SSEA-1 Polyclonal Antibody, Unconjugated at 1:300 overnight at 4˚C. (CD15 antibody (AA 251-295))

4 images

(2 references)

Catalog No. ABIN733193

Datasheet as PDF

Target See all CD15 (FUT4) Antibodies

CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
Binding Specificity
16

15

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 251-295
Reactivity
149

50

31

2

1
Human, Mouse, Rat
Host
86

64
Rabbit
Compatible Secondaries
Clonality
97

52

1
Polyclonal
Conjugate
55

11

9

8

5

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD15 antibody is un-conjugated
Application
114

47

36

32

18

16

15

12

12

10

7

5

4

3

3

2

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse CD15

Isotype

IgG

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FUT4 Reactivity: Mouse IHC, WB, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody
FUT4 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-158 unconjugated

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 199-302) antibody
FUT4 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 6B11B4 unconjugated

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 1-147) antibody
FUT4 Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 6C5A3 unconjugated

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 200-300) antibody
FUT4 Reactivity: Human IHC, IF, WB Host: Rabbit Polyclonal unconjugated

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (FITC)
FUT4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-158 FITC

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (Biotin)
FUT4 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-158 Biotin

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (APC)
FUT4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-158 APC

anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody
FUT4 Reactivity: Human IHC, ELISA, ICC Host: Mouse Monoclonal 4-00E-10 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Cong, Cao, Liu: "Effects of different feeder layers on culture of bovine embryonic stem cell-like cells in vitro." in: Cytotechnology, (2014) (PubMed).

Zschemisch, Eisenblätter, Rudolph, Glage, Dorsch: "Immortalized tumor derived rat fibroblasts as feeder cells facilitate the cultivation of male embryonic stem cells from the rat strain WKY/Ztm." in: SpringerPlus, Vol. 3, pp. 588, (2014) (PubMed).
Target

CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name

CD15/Fut4/SSEA-1 (FUT4 Products)

Synonyms

AI451562 antibody, CD15 antibody, FAL antibody, FucT-IV antibody, LeX antibody, SSEA-1 antibody, Ssea1 antibody, ELFT antibody, FCT3A antibody, FUC-TIV antibody, FUTIV antibody, Fuct antibody, fucosyltransferase 4 antibody, Fut4 antibody, FUT4 antibody

Background

Synonyms: FAL, LeX, CD15, Ssea1, SSEA-1, FucT-IV, AI451562, Alpha-(1,3)-fucosyltransferase 4, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, Galactoside 3-L-fucosyltransferase, Fut4, Elft
Background: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Gene ID

14345

UniProt

Q11127