Calpain 2 antibody (AA 301-400)

Catalog No. ABIN731858

Product Details anti-Calpain 2 Antibody

Target: Calpain 2 (CAPN2)

Binding Specificity: AA 301-400

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Calpain 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Calpain 2

Target: Calpain 2 (CAPN2)

Alternative Name: Calpain 2 (CAPN2 Products)

Synonyms: CANP2 antibody, CANPL2 antibody, CANPml antibody, mCANP antibody, AI326419 antibody, Capa-2 antibody, Capa2 antibody, m-calpain antibody, m-calpin antibody, capn2 antibody, CAPN2 antibody, im:6908877 antibody, zgc:109906 antibody, calpain 2 antibody, calpain 2, gene2 antibody, calpain 2, (m/II) large subunit b antibody, CAPN2 antibody, Capn2 antibody, capn2.2 antibody, capn2b antibody

Background:

Synonyms: Calcium activated neutral proteinase 2, Calcium activated neutral proteinase, CALP80, Calpain 2 m/II large subunit, Calpain 2 catalytic subunit, Calpain 2 large catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, Calcium-activated neutral proteinase 2, CALP80, Calpain 2, large [catalytic] subunit, Calpain M-type, Calpain-2 catalytic subunit, Calpain-2 large subunit, CAN2_MOUSE, M-calpain, Millimolar-calpain, Calpain2, CANP 2, CANP L2, CANP2, CANPL 2, CANPL2, CANPml, Capa2, CAPN 2, CAPN2, FLJ39928, M calpain, M calpin, M type, mCANP, Millimolar calpain.

Background: Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Gene ID: 824