F-Actin antibody (AA 1-50)

Catalog No. ABIN731498

Product Details anti-F-Actin Antibody

Target: F-Actin

Binding Specificity: AA 1-50

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This F-Actin antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Chicken, Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-F-Actin antibody (ABIN731498)

Zhang, Zhao, Yu, Liu, Hao, Zhang, Ge, Min, Shen, Li, Kou, Ma, Li: "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." in: Theriogenology, Vol. 95, pp. 154-162, (2018) (PubMed).

Clark, Garcia Galindo, Patel, Parry, Stoll, Yavorski, Pinkason, Johnson, Walker, Johnson, Sexton, Coppola, Blanck: "The human, F-actin-based cytoskeleton as a mutagen sensor." in: Cancer cell international, Vol. 17, pp. 121, (2018) (PubMed).

Guo, Liu: "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 2030-2036, (2017) (PubMed).

Siar, Rahman, Tsujigiwa, Mohamed Om Alblazi, Nagatsuka, Ng: "Invadopodia proteins, cortactin, N-WASP and WIP differentially promote local invasiveness in ameloblastoma." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 45, Issue 8, pp. 591-8, (2016) (PubMed).

Chen, Chen, Lu, Liu, Jiang, Ma: "Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans." in: Andrology, Vol. 4, Issue 6, pp. 1064-1072, (2016) (PubMed).

Li, Zhang, Chen, Li, Zhang: "Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice." in: Journal of molecular histology, Vol. 47, Issue 5, pp. 485-90, (2016) (PubMed).

Murakami, Nakanishi, Hirotani, Ohni, Tang, Masuda, Enomoto, Sakurai, Amano, Yamada, Nemoto: "Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma." in: Acta histochemica et cytochemica, Vol. 49, Issue 5, pp. 131-140, (2016) (PubMed).

Yuan, Lv, Li, Gao, Song, Zhang, Xing, Kong, Wang, Li, Zhou, Gao, Xiao, Yin, Wei, He, Zhang: "Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis." in: Nature cell biology, Vol. 17, Issue 9, pp. 1169-81, (2015) (PubMed).

Du, Jiang, Xu, An, Liu, Li, Zhang: "Damage of hippocampal neurons in rats with chronic alcoholism." in: Neural regeneration research, Vol. 9, Issue 17, pp. 1610-5, (2014) (PubMed).

Zhong, Zhang, Wang, Qin: "Regulation of fibrochondrogenesis of mesenchymal stem cells in an integrated microfluidic platform embedded with biomimetic nanofibrous scaffolds." in: PLoS ONE, Vol. 8, Issue 4, pp. e61283, (2013) (PubMed).

Kang, Liu, Fan, Deng: "A potential gravity-sensing role of vascular smooth muscle cell glycocalyx in altered gravitational stimulation." in: Astrobiology, Vol. 13, Issue 7, pp. 626-36, (2013) (PubMed).

Su, Peng, Jiang, Wu, Qiao, Li, Qi: "In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF) as a novel bioactive bone screw." in: PLoS ONE, Vol. 8, Issue 7, pp. e68342, (2013) (PubMed).

Zhao, Wang, Kong, Zhang, Meng, Jiang, Wu, Yu, Jin: "Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2." in: World journal of gastroenterology : WJG, Vol. 17, Issue 9, pp. 1152-9, (2011) (PubMed).

Target Details for F-Actin

Target: F-Actin

Abstract: F-Actin Products

Background:

Synonyms: CapZ alpha-1, F-actin-capping protein subunit alpha-1, CAZA1_HUMAN

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.

Gene ID: 60

UniProt: P52907