HBSAg antibody (Biotin)

Catalog No. ABIN731305

Product Details anti-HBSAg Antibody (Biotin)

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Reactivity: Virus, Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HBSAg antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Virus

Predicted Reactivity: Bee

Purification: Purified by Protein A.

Immunogen: Human Hepatitis B Surface Antigen

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References for anti-HBSAg antibody (Biotin) (ABIN731305)

Andriamandimby, Olive, Shimakawa, Rakotomanana, Razanajatovo, Andrianinarivomanana, Ravalohery, Andriamamonjy, Rogier, Héraud: "Prevalence of chronic hepatitis B virus infection and infrastructure for its diagnosis in Madagascar: implication for the WHO's elimination strategy." in: BMC public health, Vol. 17, Issue 1, pp. 636, (2018) (PubMed).

Target Details for HBSAg

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Alternative Name: HBsAg (HBsAg Products)

Target Type: Viral Protein

Background:

Synonyms: HBsAg, HBV major surface antigen, HBV surface antigen, Hepatitis B Virus major surface antigen, Major surface antigen, S, Human Hepatitis B Surface Antigen

Background: Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

UniProt: Q67926