CD11b antibody (AA 77-180)

Catalog No. ABIN730918

Product Details anti-CD11b Antibody

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Binding Specificity: AA 77-180

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Specificity: The immunogen of this product has partial sequence similarity to CD11d and may cross react.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD11b

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD11b antibody (ABIN730918)

Yamaguchi, Sato, Kato-Itoh, Goto, Hara, Sanbo, Mizuno, Kobayashi, Yanagida, Umino, Ota, Hamanaka, Masaki, Rashid, Hirabayashi, Nakauchi: "Interspecies organogenesis generates autologous functional islets." in: Nature, Vol. 542, Issue 7640, pp. 191-196, (2017) (PubMed).

Zhang, Diao, Jia, Yuan, Thamm, Wang, Jin, Pei, Zhou, Yu, Zhao, Cheng, Du, Huang, Zhang, Lin: "Proteus mirabilis inhibits cancer growth and pulmonary metastasis in a mouse breast cancer model." in: PLoS ONE, Vol. 12, Issue 12, pp. e0188960, (2017) (PubMed).

Long, Luo, Wang, Bates, Shetty: "Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells." in: Aging and disease, Vol. 8, Issue 3, pp. 301-313, (2017) (PubMed).

Velázquez, Enos, McClellan, Cranford, Chatzistamou, Singh, Nagarkatti, Nagarkatti, Fan, Murphy: "MicroRNA-155 deletion promotes tumorigenesis in the azoxymethane-dextran sulfate sodium model of colon cancer." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 6, pp. G347-58, (2016) (PubMed).

Kobayashi, Tanizaki, Kimura, Ishida, Nosaka, Toujima, Kuninaka, Minami, Ino, Kondo: "AG490, a Jak2 inhibitor, suppressed the progression of murine ovarian cancer." in: European journal of pharmacology, Vol. 766, pp. 63-75, (2015) (PubMed).

Zhang, Hu, Liu, Ye, Gui, Zhou, Qi, He, Wang, Wang: "CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment." in: Scientific reports, Vol. 5, pp. 15948, (2015) (PubMed).

Liu, Xie, Ma, Zhang, Liang, Shi, Yu, Feng, Zhang, Wu: "Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation." in: Scientific reports, Vol. 4, pp. 4833, (2014) (PubMed).

Yu, Yang, Wang, Chen, Lu, Luo, Wang: "Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma." in: Alcoholism, clinical and experimental research, Vol. 38, Issue 10, pp. 2597-606, (2014) (PubMed).

Long, Qiu, Wang, Yang, Jia, Xin, Wang, Han, Fei, Liu: "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." in: Brain research, Vol. 1532, pp. 1-13, (2013) (PubMed).

Long, Qiu, Liu, Fei, Feng, Wang, Zhong, Yi, Liu, Zhang, Han: "Functional recovery and neuronal regeneration of a rat model of epilepsy by transplantation of Hes1-down regulated bone marrow stromal cells." in: Neuroscience, Vol. 212, pp. 214-24, (2012) (PubMed).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b, CD11c (ITGAM Products)

Synonyms: CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody

Background:

Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, ITGAM, Integrin alpha-X, ITGAX, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95, CD11c

Background: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Gene ID: 3684

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation