SIRT1 antibody (AA 101-200)

Catalog No. ABIN729853

Product Details anti-SIRT1 Antibody

Target: SIRT1 (Sirtuin 1 (SIRT1))

Binding Specificity: AA 101-200

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SIRT1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SIRT1 antibody (ABIN729853)

Zeng, Hu, He, Wang, Li, Xiong, Ye: "Hypothermic machine perfusion ameliorates inflammation during ischemia‑reperfusion injury via sirtuin‑1‑mediated deacetylation of nuclear factor‑κB p65 in rat livers donated after circulatory death." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8649-8656, (2018) (PubMed).

Yan, Jin, Cao, Wang, Lu, Yang, Wu, Cai: "Effects of Aluminium on Long-Term Memory in Rats and on SIRT1 Mediating the Transcription of CREB-Dependent Gene in Hippocampus." in: Basic & clinical pharmacology & toxicology, Vol. 121, Issue 4, pp. 342-352, (2017) (PubMed).

Hu, Fan, Lv, Zhou, Yang, Zheng, Cao: "5-Aminolevulinic acid-mediated sonodynamic therapy induces anti-tumor effects in malignant melanoma via p53-miR-34a-Sirt1 axis." in: Journal of dermatological science, Vol. 79, Issue 2, pp. 155-62, (2015) (PubMed).

Mohan, Kumar, Lackner, Alvarez: "Dysregulated miR-34a-SIRT1-acetyl p65 axis is a potential mediator of immune activation in the colon during chronic simian immunodeficiency virus infection of rhesus macaques." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 1, pp. 291-306, (2014) (PubMed).

Shi, Yin, Xuan, Yao, Meng, Hou: "Vam3, a derivative of resveratrol, attenuates cigarette smoke-induced autophagy." in: Acta pharmacologica Sinica, Vol. 33, Issue 7, pp. 888-96, (2012) (PubMed).

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1 (SIRT1 Products)

Synonyms: sirtuin 1 antibody, AA673258 antibody, SIR2L1 antibody, Sir2 antibody, Sir2a antibody, Sir2alpha antibody, sir2l1 antibody, sirtuin antibody, SIR2 antibody, sirtuin 1 antibody, sirtuin 1 L homeolog antibody, SIRT1 antibody, SRT1 antibody, Sirt1 antibody, sirt1.L antibody

Background:

Synonyms: SIR2L1, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SIRT1

Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Gene ID: 23411

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling