APOA1 antibody (AA 51-150)

Catalog No. ABIN728968

Product Details anti-APOA1 Antibody

Target: APOA1 (Apolipoprotein A-I (APOA1))

Binding Specificity: AA 51-150

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APOA1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-APOA1 antibody (ABIN728968)

Zhang, Zhao, Yu, Liu, Hao, Zhang, Ge, Min, Shen, Li, Kou, Ma, Li: "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." in: Theriogenology, Vol. 95, pp. 154-162, (2018) (PubMed).

Wu, Wu, Liu, Wang, Na, Wang, Li: "Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers." in: Proteome science, Vol. 14, Issue 1, pp. 9, (2016) (PubMed).

Hibert, Prunier-Mirebeau, Beseme, Chwastyniak, Tamareille, Lamon, Furber, Pinet, Prunier: "Apolipoprotein a-I is a potential mediator of remote ischemic preconditioning." in: PLoS ONE, Vol. 8, Issue 10, pp. e77211, (2013) (PubMed).

Liu, Zhao, Lu, Fan, Wang: "Proteomic study on usnic-acid-induced hepatotoxicity in rats." in: Journal of agricultural and food chemistry, Vol. 60, Issue 29, pp. 7312-7, (2012) (PubMed).

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1 (APOA1 Products)

Background:

Synonyms: Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

Background: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Gene ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism