Nanog antibody (AA 71-170)

Catalog No. ABIN728758

Product Details anti-Nanog Antibody

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Binding Specificity: AA 71-170

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Nanog

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Nanog antibody (ABIN728758)

El-Badawy, Ghoneim, Gabr, Salah, Mohamed, Amer, El-Badri: "Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 254, (2017) (PubMed).

Liu, Zhang, Wang, Yang, Tan: "JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells." in: Acta pharmacologica Sinica, Vol. 36, Issue 9, pp. 1099-106, (2016) (PubMed).

Liu, Li, Liu, Sui, Liu, Xiao, Zheng, Chen, Yao, Xing, Zhou, Li: "A signature for induced pluripotent stem cell-associated genes in colorectal cancer." in: Medical oncology (Northwood, London, England), Vol. 30, Issue 1, pp. 426, (2013) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog (NANOG Products)

Synonyms: 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody

Background:

Synonyms: ENK, ecat4, 2412E2Rik, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog, Nanog

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Gene ID: 71950

UniProt: Q80Z64

Pathways: Stem Cell Maintenance