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NFASC antibody (AA 501-650)

Name: NFASC antibody (AA 501-650)
SKU: ABIN727325
Price: 384.62 USD
Availability: InStock

NFASC Reactivity: Human, Rat, Mouse WB, ELISA, IF (p), IF (cc), ICC, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

Mouse cerebellum lysates probed with Neurofascin Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (NFASC antibody (AA 501-650))

Formalin-fixed and paraffin embedded: rat spinal cord tissue labeled with Anti-Neurofascin Polyclonal Antibody, Unconjugated (ABIN727325) at 1:200, followed by conjugation to the secondary antibody and DAB staining (NFASC antibody (AA 501-650))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Neurofascin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (NFASC antibody (AA 501-650))

IHC (p)

Formalin-fixed and paraffin embedded: rat spinal cord tissue labeled with Anti-Neurofascin Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (NFASC antibody (AA 501-650))

IHC (p)

4 images

Catalog No. ABIN727325

Datasheet as PDF

Target See all NFASC Antibodies

NFASC (Neurofascin (NFASC))
Binding Specificity
14

2

1

1

1

1

1

1

1

1

1
AA 501-650
Reactivity
29

24

24

1
Human, Rat, Mouse
Host
25

3

1

1
Rabbit
Compatible Secondaries
Clonality
28

2
Polyclonal
Conjugate
10

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This NFASC antibody is un-conjugated
Application
24

12

12

6

5

2

2

2

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Horse,Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Neurofascin-155

Isotype

IgG
anti-Neurofascin (NFASC) (AA 830-1100) antibody
NFASC Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Neurofascin (NFASC) (C-Term) antibody
NFASC Reactivity: Human, Rat, Mouse WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Neurofascin (NFASC) (AA 661-758) antibody
NFASC Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Neurofascin (NFASC) (AA 1142-1155), (C-Term), (Intracellular) antibody
NFASC Reactivity: Human, Rat, Mouse WB Host: Rabbit Polyclonal unconjugated

anti-Neurofascin (NFASC) (N-Term) antibody
NFASC Reactivity: Human, Rat, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Neurofascin (NFASC) (AA 760-1007) antibody
NFASC Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Neurofascin (NFASC) (AA 501-650) antibody (Biotin)
NFASC Reactivity: Human, Rat, Mouse WB, ELISA, IHC (fro), IHC (p) Host: Rabbit Polyclonal Biotin

anti-Neurofascin (NFASC) (AA 501-650) antibody (AbBy Fluor® 680)
NFASC Reactivity: Human, Rat, Mouse WB, IF (p), IF (cc) Host: Rabbit Polyclonal AbBy Fluor® 680

anti-Neurofascin (NFASC) (AA 501-650) antibody (AbBy Fluor® 555)
NFASC Reactivity: Human, Rat, Mouse WB, IF (p), IF (cc) Host: Rabbit Polyclonal AbBy Fluor® 555

anti-Neurofascin (NFASC) (AA 501-650) antibody (AbBy Fluor® 647)
NFASC Reactivity: Human, Rat, Mouse WB, IF (p), IF (cc) Host: Rabbit Polyclonal AbBy Fluor® 647

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

NFASC (Neurofascin (NFASC))

Alternative Name

Neurofascin (NFASC Products)

Synonyms

sc:d0205 antibody, NF antibody, NRCAML antibody, AA387016 antibody, D430023G06Rik antibody, mKIAA0756 antibody, neurofascin antibody, neurofascin homolog (chicken) a antibody, NFASC antibody, nfasca antibody, Nfasc antibody

Background

Synonyms: NF, NRCAML, Neurofascin, NFASC, KIAA0756, Neurofascin-155, NF-155
Background: Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.

Gene ID

23114

UniProt

O94856

Pathways

Cell-Cell Junction Organization