14-3-3 Pan (AA 174-245) antibody

Catalog No. ABIN726710

Product Details

Target: 14-3-3 Pan

Binding Specificity: AA 174-245

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae

Predicted Reactivity: Sheep,Drosophila

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human 14-3-3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: 14-3-3 Pan

Alternative Name: 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon

Background:

Synonyms: HS1, GW128, YWHAA, KCIP-1, HEL-S-1, 14-3-3 protein beta/alpha, Protein 154, Protein kinase C inhibitor protein 1, YWHAB

Background: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

Gene ID: 7529

UniProt: P31946