FASL antibody (AA 196-281)

Catalog No. ABIN726410

Product Details anti-FASL Antibody

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Binding Specificity: AA 196-281

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Fas Ligand

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FASL antibody (ABIN726410)

Lv, Zheng, Zhou, Jia, Wang, Liu, Zhao, Ji, Li, Cao: "Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma." in: Scientific reports, Vol. 7, pp. 40967, (2017) (PubMed).

Wang, Zhang, Yang, Luo, Zhang, Wang: "Lipopolysaccharide-induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro-inflammatory cytokines and miR-187." in: Molecular reproduction and development, (2015) (PubMed).

Song, Li, Shi, Lehmler, Fu, Su, Xia, Song, Song: "Polychlorinated Biphenyl Quinone Metabolite Promotes p53-Dependent DNA Damage Checkpoint Activation, S-Phase Cycle Arrest and Extrinsic Apoptosis in Human Liver Hepatocellular Carcinoma HepG2 Cells." in: Chemical research in toxicology, Vol. 28, Issue 11, pp. 2160-9, (2015) (PubMed).

Peng, Fu, Guo, Zhang, Di, Jiang, Li: "Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells I n -v itro." in: Iranian journal of pharmaceutical research : IJPR, Vol. 14, Issue 1, pp. 251-61, (2015) (PubMed).

Qi, Fu, Wang, Liu, Quan, Kourouma, Yan, Yu, Duan, Yang: "BPA-induced apoptosis of rat Sertoli cells through Fas/FasL and JNKs/p38 MAPK pathways." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 50, pp. 108-16, (2014) (PubMed).

Chen, Xie, Xue, Ji, Chang, Ma, Bi: "Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius." in: Research in veterinary science, Vol. 94, Issue 3, pp. 496-503, (2013) (PubMed).

Shi, Song, Wang, Wang, Liang, Hu, Yu, Guan, Cheng, Yang: "β-Benzene hexachloride induces apoptosis of rat Sertoli cells through generation of reactive oxygen species and activation of JNKs and FasL." in: Environmental toxicology, Vol. 26, Issue 2, pp. 124-35, (2011) (PubMed).

Shi, Song, Wang, Liang, Hu, Guan, Cheng, Yang: "p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway." in: Journal of biomedicine & biotechnology, Vol. 2009, pp. 181282, (2009) (PubMed).

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: Fas Ligand (FASL Products)

Synonyms: ALPS1B antibody, APT1LG1 antibody, APTL antibody, CD178 antibody, CD95-L antibody, CD95L antibody, FASL antibody, TNFSF6 antibody, fasl antibody, Fas-L antibody, Faslg antibody, Tnfsf6 antibody, gld antibody, Apt1Lg1 antibody, Fasl antibody, FASLG antibody, zgc:162027 antibody, Fas ligand antibody, Fas ligand L homeolog antibody, Fas ligand (TNF superfamily, member 6) antibody, FASLG antibody, faslg.L antibody, Fasl antibody, Faslg antibody, faslg antibody

Background:

Synonyms: APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, Fas ligand, FASLG

Background: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.

Gene ID: 356

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity