GFAP antibody (AA 51-150)

Catalog No. ABIN726200

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Binding Specificity: AA 51-150

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GFAP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GFAP antibody (ABIN726200)

Farah, Siwek, Cummings: "Tau accumulations in the brains of woodpeckers." in: PLoS ONE, Vol. 13, Issue 2, pp. e0191526, (2018) (PubMed).

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Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, (2017) (PubMed).

Sweeney, Qi, Xu, Yang: "Activation of hypothalamic astrocytes suppresses feeding without altering emotional states." in: Glia, Vol. 64, Issue 12, pp. 2263-2273, (2016) (PubMed).

Yan, Li, Li, Lin, Yang: "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." in: Neurochemical research, (2016) (PubMed).

Zhao, Sun, Miao, Ji, Zhao, Sun, Miao, Yoshii, Mizushima, Wang, Zhang: "The autophagy gene Wdr45/Wipi4 regulates learning and memory function and axonal homeostasis." in: Autophagy, Vol. 11, Issue 6, pp. 881-90, (2015) (PubMed).

Mori, Matsubara, Matsubara, Uchikura, Hashimoto, Fujioka, Matsumoto: "Stromal Cell-Derived Factor-1? Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 18018-32, (2015) (PubMed).

Shan, Huang, You, Yuan, Gao, Hu, Du: "High efficiency intracellular transport of cationic peptide stearate for gene delivery in tumor cells and multipotent stem cells." in: Journal of biomedical nanotechnology, Vol. 10, Issue 11, pp. 3231-43, (2015) (PubMed).

Liang, Sun, Yu, He, Xie: "Scorpion ethanol extract and valproic acid effects on hippocampal glial fibrillary acidic protein expression in a rat model of chronic-kindling epilepsy induced by lithium chloride-pilocarpine." in: Neural regeneration research, Vol. 7, Issue 6, pp. 426-33, (2015) (PubMed).

Fan, Hu, Ji, Sun, Xiong, Yang, Liu: "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 4, pp. 304-12, (2014) (PubMed).

Xiang, Wei, Chen, Liu, Liu, Wang, Zhang: "Anti-inflammatory effect of acetylpuerarin on eicosanoid signaling pathway in primary rat astrocytes." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 4, pp. 577-85, (2014) (PubMed).

Ma, Cao, Li, Gao, Qi, Zhao, Du, Xue, Peng, Wen, Chen, Ning, Huang, Zhang, Gao, Yu, Chen: "Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation." in: Cell research, Vol. 24, Issue 8, pp. 912-24, (2014) (PubMed).

Du, Liu, Chen, Dou, Lei, Chang, Cai, Cui, Yang, Sun, Li, Jiang: "Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness." in: Neuro-oncology, (2014) (PubMed).

Zuo, Wang, Li, Lin, Duan, Qi, Li, Sun, Wu: "Existence of glia mitigated ketamine-induced neurotoxicity in neuron-glia mixed cultures of neonatal rat cortex and the glia-mediated protective effect of 2-PMPA." in: Neurotoxicology, Vol. 44, pp. 218-30, (2014) (PubMed).

Hu, Zheng, Jia, Li, Dong, Wang, Lin, Zhang, Zhao, Xu, Zhou, Yuan, Zhang, Ren: "Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation." in: Clinical and experimental immunology, Vol. 175, Issue 2, pp. 268-84, (2014) (PubMed).

Zhao, Zhao, Wang, Xu, Miao, Feng, Chen, Kovács, Fan, Zhang: "Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 731-41, (2013) (PubMed).

Liu, Xue, Tang, Hou, Qi, Chen, Chen, Zhang, Chen, Xu: "A simple method for isolating and culturing the rat brain microvascular endothelial cells." in: Microvascular research, Vol. 90, pp. 199-205, (2013) (PubMed).

Xiang, Chen, Liu, Liu, Wei, Sun, Wang, Zhang: "Inhibition of sPLA?-IIA prevents LPS-induced neuroinflammation by suppressing ERK1/2-cPLA?? pathway in mice cerebral cortex." in: PLoS ONE, Vol. 8, Issue 10, pp. e77909, (2013) (PubMed).

Zhao, Zhao, Miao, Wang, Sun, Zhang: "The p53-induced gene Ei24 is an essential component of the basal autophagy pathway." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42053-63, (2012) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

Background:

Synonyms: Glial fibrillary acidic protein, GFAP

Background: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Gene ID: 2670

UniProt: P14136