HSPD1 antibody (AA 501-573)

Catalog No. ABIN726080

Product Details anti-HSPD1 Antibody

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Binding Specificity: AA 501-573

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HSP60

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HSPD1 antibody (ABIN726080)

Liu, Cao, Li, Wang, Zhang, Dong Zhang, Liu, Yuan, Zhan: "Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination." in: Cell death and differentiation, Vol. 24, Issue 4, pp. 683-693, (2017) (PubMed).

Kengkoom, Ampawong: "Chronic ingestion of high dosed Phikud Navakot extraction induces mesangiolysis in rats with alteration of AQP1 and Hsp60 expressions." in: BioMed research international, Vol. 2015, pp. 462387, (2015) (PubMed).

Isarangkul, Wiyakrutta, Kengkoom, Reamtong, Ampawong: "Mitochondrial and cytoskeletal alterations are involved in the pathogenesis of hydronephrosis in ICR/Mlac-hydro mice." in: International journal of clinical and experimental medicine, Vol. 8, Issue 6, pp. 9192-204, (2015) (PubMed).

Qi, Chen, Chan, Li, Yuan, Yu, Lin, Yew, Kung, Lai: "Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma." in: International journal of cancer, Vol. 122, Issue 12, pp. 2682-8, (2008) (PubMed).

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSP60 (HSPD1 Products)

Background:

Synonyms: HLD4, CPN6, GROEL, HSP6, HSP65, SPG13, HSP-6, HuCHA6, 6 kDa heat shock protein, mitochondrial, 6 kDa chaperonin, Chaperonin 6, Heat shock protein 6, Mitochondrial matrix protein P1, P6 lymphocyte protein, HSPD1

Background: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Gene ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity