Actin antibody

Catalog No. ABIN726050

Product Details anti-Actin Antibody

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Dog, Cow, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Pig,Chicken,Cat,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human alpha Actin

Isotype: IgG

Application Details

Application Notes: WB(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Actin antibody (ABIN726050)

Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." in: PLoS ONE, Vol. 9, Issue 6, pp. e99802, (2014) (PubMed).

Wang, Liao: "Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue." in: BMC urology, Vol. 14, pp. 69, (2014) (PubMed).

Song, Yan, An, Hao, Guo, Zhang, Li, Li, Sun: "Potential contribution of SOCC to cerebral vasospasm after experimental subarachnoid hemorrhage in rats." in: Brain research, Vol. 1517, pp. 93-103, (2013) (PubMed).

Lv, Fan, Su: "Is a swine model of arteriovenous malformation suitable for human extracranial arteriovenous malformation? A preliminary study." in: Cardiovascular and interventional radiology, Vol. 36, Issue 5, pp. 1364-70, (2013) (PubMed).

Liu, Xue, Tang, Hou, Qi, Chen, Chen, Zhang, Chen, Xu: "A simple method for isolating and culturing the rat brain microvascular endothelial cells." in: Microvascular research, Vol. 90, pp. 199-205, (2013) (PubMed).

Han, Liu, Li: "Salvianolic acid B inhibits autophagy and protects starving cardiac myocytes." in: Acta pharmacologica Sinica, Vol. 32, Issue 1, pp. 38-44, (2011) (PubMed).

Xu, Chen, Huang, Varghese, Moorhead, Yan, Powis, Li, Ruan: "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." in: American journal of physiology. Renal physiology, Vol. 301, Issue 4, pp. F713-22, (2011) (PubMed).

Waladali, Luo, Li, Zheng, Hu: "17Beta-estradiol affects the proliferation and apoptosis of rat bladder neck smooth muscle cells by modulating cell cycle transition and related proteins." in: World journal of urology, Vol. 27, Issue 2, pp. 241-8, (2009) (PubMed).

Target Details for Actin

Target: Actin

Abstract: Actin Products

Background:

Synonyms: Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Molecular Weight: 42kDa

Gene ID: 58