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Leptin antibody (AA 101-154)

Name: Leptin antibody (AA 101-154)
SKU: ABIN725030
Price: 384.62 USD
Availability: InStock

LEP Reactivity: Mouse, Rat ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded: rat liver labeled with Anti-Leptin Polyclonal Antibody (ABIN725030), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining (Leptin antibody (AA 101-154))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin antibody (AA 101-154))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse liver, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Leptin antibody (AA 101-154))

IHC (p)

3 images

Catalog No. ABIN725030

Datasheet as PDF

Target See all Leptin (LEP) Antibodies

Leptin (LEP)
Binding Specificity
25

15

15

15

12

12

7

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-154
Reactivity
122

73

71

12

8

7

5

3

3

3

2

1

1

1
Mouse, Rat
Host
161

29

8

5

1
Rabbit
Compatible Secondaries
Clonality
173

30
Polyclonal
Conjugate
98

24

16

8

8

7

3

3

3

3

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1
This Leptin antibody is un-conjugated
Application
127

83

59

38

38

37

32

30

22

18

15

11

10

7

5

4

2

2

2

1

1

1

1

1

1
ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse Leptin

Isotype

IgG

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anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 17-100) antibody
LEP Reactivity: Human, Mouse, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 8-37) antibody
LEP Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 29-160) antibody
LEP Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 7-37), (N-Term) antibody
LEP Reactivity: Human WB, IF, FACS, EIA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C5 unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Dog WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Leptin (LEP)

Alternative Name

Leptin (LEP Products)

Synonyms

ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody

Background

Synonyms: ob, obese, Leptin, Obesity factor, Lep
Background: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Gene ID

16846

UniProt

P41160

Pathways

JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process