MUC16 antibody (AA 250-350)

Catalog No. ABIN724760

Product Details anti-MUC16 Antibody

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Binding Specificity: AA 250-350

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CA125

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MUC16 antibody (ABIN724760)

Guo, Kenney, Muller, Adams, Rutledge, Romero, Murray-Krezan, Prekeris, Sklar, Hudson, Wandinger-Ness: "R-ketorolac Targets Cdc42 and Rac1 and Alters Ovarian Cancer Cell Behaviors Critical for Invasion and Metastasis." in: Molecular cancer therapeutics, (2015) (PubMed).

Zong, Wu, Xu, Ju, Yan: "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." in: Biosensors & bioelectronics, Vol. 43, pp. 372-8, (2013) (PubMed).

Zong, Wu, Wang, Ju, Yan: "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." in: Analytical chemistry, Vol. 84, Issue 5, pp. 2410-5, (2012) (PubMed).

Xie, Dong, Ren: "Study on homogeneous competitive immune reaction by fluorescence correlation spectroscopy: using synthetic peptide as antigen." in: Talanta, Vol. 79, Issue 3, pp. 971-4, (2009) (PubMed).

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125 (CA125 Products)

Background:

Synonyms: CA125, Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125, MUC16

Background: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Gene ID: 94025

UniProt: Q8WXI7