Ascorbate Oxidase antibody (HRP)
AO
Reactivity: Pumpkin
ELISA, WB, IP
Host: Rabbit
Polyclonal
HRP
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- Target See all Ascorbate Oxidase (AO) products
- Ascorbate Oxidase (AO)
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Reactivity
- Pumpkin
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Ascorbate Oxidase antibody is conjugated to HRP
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Application
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Ascorbate Oxidase Antibody Peroxidase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].
- Characteristics
- Synonyms: rabbit anti-Ascorbate Oxidase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Ascorbate Oxidase Antibody, L-ascorbate oxidase, Ascorbase, ASO
- Purification
- Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein
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- Application Notes
- Application Note: Anti-Ascorbate Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:3,000 - 1:10,000 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target
- Ascorbate Oxidase (AO)
- Alternative Name
- AAO (AO Products)
- Background
- Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.
- UniProt
- P24792
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