Uricase (ABC3735) antibody (HRP)

Catalog No. ABIN7565853

Product Details

Target: Uricase (ABC3735)

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Purpose: Uricase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].

Characteristics: Synonyms: goat anti-uricase antibody, HRP conjugated goat anti-uricase antibody, Urate oxidase antibody

Purification: Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Uricase antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below. Western Blot Dilution: 1:500 - 1:1,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Uricase (ABC3735)

Alternative Name: ABC3735

Background: Background: Anti-Uricase antibody detects uricase. Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localized in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form, and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. Anti-Uricase antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.

Gene ID: 3202700

NCBI Accession: YP_177228

UniProt: Q5WBJ3