Caspase 3 antibody (AA 60-100)

Catalog No. ABIN724625

Product Details anti-Caspase 3 Antibody

Target: Caspase 3 (CASP3)

Binding Specificity: AA 60-100

Reactivity: Human, Mouse, Rat, Cow, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Chicken, Cow, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Caspase 3(Active)

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Caspase 3 antibody (ABIN724625)

Zhang, Wang, Li, Yang, Li, Wang: "The anti-carcinogenesis properties of erianin in the modulation of oxidative stress-mediated apoptosis and immune response in liver cancer." in: Aging, Vol. 11, Issue 22, pp. 10284-10300, (2020) (PubMed).

Li, Li, Xi, Li, Ding, Li: "The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway." in: Lasers in medical science, Vol. 34, Issue 3, pp. 607-614, (2019) (PubMed).

Chu, Wang, Tang, Shi, Gao, Ma, Lu, Han, Jia, Wang, Adam, Liu, Xiao, Wang, Yang: "Newcastle Disease Virus V Protein Inhibits Cell Apoptosis and Promotes Viral Replication by Targeting CacyBP/SIP." in: Frontiers in cellular and infection microbiology, Vol. 8, pp. 304, (2019) (PubMed).

Chen, Wang, Chen, Zhang: "Conditional deletion of Bmp2 in cranial neural crest cells recapitulates Pierre Robin sequence in mice." in: Cell and tissue research, Vol. 376, Issue 2, pp. 199-210, (2019) (PubMed).

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Wang, Chu, Liu, Pang, Gao, Tang, Ma, Lu, Adam, Dang, Xiao, Wang, Yang: "Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1." in: Veterinary research, Vol. 49, Issue 1, pp. 102, (2018) (PubMed).

Zheng, Bi, Yang, Zhao, Wang, Li, Zhou: "Hyperglycemia Induced by Chronic Restraint Stress in Mice Is Associated With Nucleus Tractus Solitarius Injury and Not Just the Direct Effect of Glucocorticoids." in: Frontiers in neuroscience, Vol. 12, pp. 983, (2018) (PubMed).

Abdel-Hamid, El-Azab, Moustafa: "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 390, Issue 4, pp. 379-395, (2017) (PubMed).

Ochigbo, Saba, Oyagbemi, Omobowale, Asenuga: "Polyphenol-rich fraction of Parquetina nigrescens mitigates dichlorvos-induced neurotoxicity and apoptosis." in: Journal of Ayurveda and integrative medicine, Vol. 8, Issue 1, pp. 27-36, (2017) (PubMed).

Kubatka, Uramova, Kello, Kajo, Kruzliak, Mojzis, Vybohova, Adamkov, Jasek, Lasabova, Zubor, Fialova, Dokupilova, Solar, Pec, Adamicova, Danko, Adamek, Busselberg: "Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma." in: Journal of cellular and molecular medicine, (2017) (PubMed).

Wang, Xuan, Cui, Wang, Chen, Wei, Zhao: "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." in: PLoS ONE, Vol. 12, Issue 4, pp. e0173469, (2017) (PubMed).

He, Chen, Yuan, Zhao, Chen, Wang, Li: "Tumor pH-Responsive Release of Drug-Conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy." in: ACS applied materials & interfaces, Vol. 9, Issue 38, pp. 32534-32544, (2017) (PubMed).

Li, Shi, Zhao, Li: "Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells." in: Oncotarget, Vol. 8, Issue 54, pp. 92855-92863, (2017) (PubMed).

Wu, Ye, Zhou, Wang, Xie, Zhang, Gao, Zhang, Zhou, Zhuang, Liu, Hang, Shi: "Roles of Pannexin-1 Channels in Inflammatory Response through the TLRs/NF-Kappa B Signaling Pathway Following Experimental Subarachnoid Hemorrhage in Rats." in: Frontiers in molecular neuroscience, Vol. 10, pp. 175, (2017) (PubMed).

Xu, Sun, Ren, Yang, Tian, Peng: "Resveratrol controlled the fate of porcine pancreatic stem cells through the Wnt/β-catenin signaling pathway mediated by Sirt1." in: PLoS ONE, Vol. 12, Issue 10, pp. e0187159, (2017) (PubMed).

Huang, Fan, Qiu: "TM4SF1 Promotes Proliferation, Invasion, and Metastasis in Human Liver Cancer Cells." in: International journal of molecular sciences, Vol. 17, Issue 5, (2017) (PubMed).

Clark, Coury, Meilhac, Petty: "WO3/Pt nanoparticles are NADPH oxidase biomimetics that mimic effector cells in vitro and in vivo." in: Nanotechnology, Vol. 27, Issue 6, pp. 65101, (2016) (PubMed).

Kubatka, Kello, Kajo, Kruzliak, Výbohová, Mojžiš, Adamkov, Fialová, Veizerová, Zulli, Péč, Statelová, Grančai, Büsselberg: "Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model." in: European journal of nutrition, (2016) (PubMed).

Yoshinaga, Kajiya, Oishi, Kamada, Ikeda, Chigwechokha, Kibe, Kishida, Kishida, Komatsu, Shiozaki: "NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation." in: European journal of pharmacology, Vol. 782, pp. 21-9, (2016) (PubMed).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase 3 (CASP3 Products)

Synonyms: CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody

Background:

Synonyms: CPP32, SCA-1, CPP32B, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, CASP3

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation