Caspase 9 antibody (AA 11-120)

Catalog No. ABIN724160

Product Details anti-Caspase 9 Antibody

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Binding Specificity: AA 11-120

Reactivity: Human, Mouse, Rat, Chicken, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Chicken, Dog, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Caspase 9 subunit p35

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Caspase 9 antibody (ABIN724160)

Wang, Xuan, Cui, Wang, Chen, Wei, Zhao: "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." in: PLoS ONE, Vol. 12, Issue 4, pp. e0173469, (2017) (PubMed).

Huang, Fan, Qiu: "TM4SF1 Promotes Proliferation, Invasion, and Metastasis in Human Liver Cancer Cells." in: International journal of molecular sciences, Vol. 17, Issue 5, (2017) (PubMed).

Wang, Wang, Huang, Yang, Zhao, Wei, Zhao: "Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways." in: Heart and vessels, (2016) (PubMed).

Yuan, Wen, Zhang, Fan: "Cytotoxicity of pilocarpine to human corneal stromal cells and its underlying cytotoxic mechanisms." in: International journal of ophthalmology, Vol. 9, Issue 4, pp. 505-11, (2016) (PubMed).

Pan, Yang, Wang, Wang, Wang, Zhou, Yin, Zhang, Zhao: "C-Abl tyrosine kinase mediates neurotoxic prion peptide-induced neuronal apoptosis via regulating mitochondrial homeostasis." in: Molecular neurobiology, Vol. 49, Issue 2, pp. 1102-16, (2014) (PubMed).

Zhao, Xu, Yun, Zhao, Li, Gong, Yuan, Yan, Zhang, Ding, Wang, Zhang, Dong, Xiu, Yang, Liu, Xue, Li: "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." in: Basic research in cardiology, Vol. 109, Issue 5, pp. 427, (2014) (PubMed).

Wang, Lu, Dai, Zhang, Wang, Wei: "Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], (2014) (PubMed).

Wang, Han, Zhou, Wu, Wang, Jia, Lv, Cheng, Pan, Liu, Zhou, Sun: "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 1, pp. 28-42, (2013) (PubMed).

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase 9 (CASP9 Products)

Synonyms: CASP9 antibody, AI115399 antibody, APAF-3 antibody, AW493809 antibody, CASP-9 antibody, Caspase-9 antibody, ICE-LAP6 antibody, Mch6 antibody, Apaf3 antibody, Casp-9-CTD antibody, Casp9_v1 antibody, Ice-Lap6 antibody, APAF3 antibody, MCH6 antibody, PPP1R56 antibody, caspase 9 antibody, CASP9 antibody, Casp9 antibody

Background:

Synonyms: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP9

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Gene ID: 842

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity