p53 antibody (AA 251-310)

Catalog No. ABIN723935

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: AA 251-310

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human P53

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-p53 antibody (ABIN723935)

Uchiumi, Shoji, Sasaki, Sasaki, Sasaki, Oyama, Sugisawa, Tanuma: "Characterization of the 5'-flanking region of the human TP53 gene and its response to the natural compound, Resveratrol." in: Journal of biochemistry, Vol. 159, Issue 4, pp. 437-47, (2016) (PubMed).

Wang, Liu, Liu, Chen, Liu, Kuang: "Hypoxia Inhibits Myogenic Differentiation through p53 Protein-dependent Induction of Bhlhe40 Protein." in: The Journal of biological chemistry, Vol. 290, Issue 50, pp. 29707-16, (2015) (PubMed).

Hong, Shen, Liu, Li, Huang, Shi: "Developmental toxicity of three hexabromocyclododecane diastereoisomers in embryos of the marine medaka Oryzias melastigma." in: Marine pollution bulletin, Vol. 101, Issue 1, pp. 110-8, (2015) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).

Carry, Banssillon: "[Intra-abdominal pressure]." in: Annales francaises d'anesthesie et de reanimation, Vol. 13, Issue 3, pp. 381-99, (1995) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: P53 proteinwt-p53 (TP53 Products)

Synonyms: BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody

Background:

Synonyms: P53, BCC7, LFS1, TRP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53

Background: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Gene ID: 7157

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect