Cytochrome C antibody (AA 51-105)

Catalog No. ABIN723590

Product Details anti-Cytochrome C Antibody

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Binding Specificity: AA 51-105

Reactivity: Human, Rat, Mouse, Rabbit, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Chicken, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow,Pig,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cytochrome C

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cytochrome C antibody (ABIN723590)

Liu, Chen, Du, Wu, Dong, Qu, Fan, Yao: "GluR3B Ab's induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction." in: Brain research bulletin, Vol. 130, pp. 60-66, (2017) (PubMed).

Abdel-Hamid, El-Azab, Moustafa: "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 390, Issue 4, pp. 379-395, (2017) (PubMed).

Tian, Wang, Zhang, Luo, Jiang, Zhang, Mei, Wu, Wu, Peng, Long, Luo, Guo: "Phosphoprotein Gene Contributes to the Enhanced Apoptosis Induced by Wild-Type Rabies Virus GD-SH-01 In Vitro." in: Frontiers in microbiology, Vol. 8, pp. 1697, (2017) (PubMed).

Gu, Chen, Jiang, Zhang: "ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction underlie apoptosis induced by resveratrol and arsenic trioxide in A549 cells." in: Chemico-biological interactions, Vol. 245, pp. 100-9, (2016) (PubMed).

Duan, Li, Li, Xing, Zhang, Li, Zhao, Sun, Gao, Li et al.: "Antioxidant tert-butylhydroquinone ameliorates arsenic-induced intracellular damages and apoptosis through induction of Nrf2-dependent antioxidant responses as well as stabilization of anti-apoptotic ..." in: Free radical biology & medicine, Vol. 94, pp. 74-87, (2016) (PubMed).

Zhang, He, Guo, Li, Wang, Yang, Cui: "Sulfiredoxin-1 protects against simulated ischaemia/reperfusion injury in cardiomyocyte by inhibiting PI3K/AKT-regulated mitochondrial apoptotic pathways." in: Bioscience reports, Vol. 36, Issue 2, (2016) (PubMed).

Gu, Chen, Jiang, Zhang: "Resveratrol Synergistically Triggers Apoptotic Cell Death with Arsenic Trioxide via Oxidative Stress in Human Lung Adenocarcinoma A549 Cells." in: Biological trace element research, Vol. 163, Issue 1-2, pp. 112-23, (2015) (PubMed).

Gao, Yu, Yan, Li, Shen, Jia, Guan, Gao, Kan: "Poly (ADP?ribose) polymerase? and cytochrome c?mediated apoptosis induces hepatocyte injury in a rat model of hyperammonia?induced hepatic failure." in: Molecular medicine reports, (2015) (PubMed).

Li, Li, Sun, Ni, Chen, Guo, Chen: "Increased Expression of Mitochondrial Inner-Membrane Protein Mpv17 After Intracerebral Hemorrhage in Adult Rats." in: Neurochemical research, Vol. 40, Issue 8, pp. 1620-30, (2015) (PubMed).

Chen, Gu, Jiang, Zhang: "Nuclear translocation of nuclear factor kappa B is regulated by G protein signaling pathway in arsenite-induced apoptosis in HBE cell line." in: Environmental toxicology, (2015) (PubMed).

Ye, Wu, Li, Li, Zheng, Liu, Ye: "Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca (2+)." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 464161, (2015) (PubMed).

Zhang, Ma, Qi, Wei, Zhang, Li, Chiu: "P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis." in: Oncotarget, Vol. 6, Issue 2, pp. 789-801, (2015) (PubMed).

Jiang, Ren, Jiang, Wang, Zhang, Yin, Wang, Qi, Wang, Feng: "Guanabenz delays the onset of disease symptoms, extends lifespan, improves motor performance and attenuates motor neuron loss in the SOD1 G93A mouse model of amyotrophic lateral sclerosis." in: Neuroscience, Vol. 277, pp. 132-8, (2014) (PubMed).

Chen, Jiang, Lai, Liu, Zhang: "Resveratrol protects against arsenic trioxide-induced oxidative damage through maintenance of glutathione homeostasis and inhibition of apoptotic progression." in: Environmental and molecular mutagenesis, (2014) (PubMed).

Fang, Zhang, Qi, Fan, Luo, Liu, Tan: "Evodiamine induces G2/M arrest and apoptosis via mitochondrial and endoplasmic reticulum pathways in H446 and H1688 human small-cell lung cancer cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e115204, (2014) (PubMed).

Liao, Li, Li, Liu: "Organellar proteome analyses of ricin toxin-treated HeLa cells." in: Toxicology and industrial health, (2014) (PubMed).

Wang, Wang, Shan, Song, Dai, Wang, Chi: "Mu-calpain mediates hippocampal neuron death in rats after lithium-pilocarpine-induced status epilepticus." in: Brain research bulletin, Vol. 76, Issue 1-2, pp. 90-6, (2008) (PubMed).

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome C (CYCS Products)

Synonyms: CYC antibody, HCS antibody, THC4 antibody, cyct antibody, cyc antibody, hcs antibody, CYCS antibody, cytc antibody, CYCSA antibody, CG17903 antibody, Cyt C antibody, Cyt c antibody, Cyt-c2 antibody, CytC-2 antibody, Cytc antibody, Cytc-p antibody, DC4 antibody, DMc01 antibody, Dmel\\CG17903 antibody, cyt c antibody, cyt c-p antibody, cyt-c-p antibody, cyt-c2 antibody, cyt.c antibody, cytochrome c antibody, dc4 antibody, pDMc01 antibody, wu:fk52b01 antibody, zgc:86706 antibody, ENSMUSG00000058927 antibody, cytochrome c, somatic antibody, cytochrome c, somatic S homeolog antibody, Cytochrome c proximal antibody, cytochrome c, somatic b antibody, cytochrome c, somatic pseudogene antibody, cytochrome c-like antibody, cytochrome c antibody, CYCS antibody, cycs.S antibody, cycs antibody, Cycs antibody, Cyt-c-p antibody, cycsb antibody, Gm10053 antibody, LOC100724240 antibody, CYC antibody, LOC100847700 antibody, LOC101107954 antibody, LOC106829744 antibody, LOC106992390 antibody, LOC102085712 antibody

Background:

Synonyms: CYC, HCS, THC4, Cytochrome c, CYCS

Background: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity