Nestin antibody (Cy3)

Catalog No. ABIN723503

Product Details anti-Nestin Antibody (Cy3)

Target: Nestin (NES)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is conjugated to Cy3

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nestin

Isotype: IgG

Application Details

Application Notes: IF(IHC-P)(1:100-500)
IF(ICC)(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Background:

Synonyms: ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, Nes.

Background: Neural Stem Cell Marker There are a certain number of neural stem cells, which can be potentially differentiated into the neurons or neuroglia, in the adult mammalian brain. These neural precursors are located in the subependymal cell layer of the ventricle. Those precursors isolated from embryonic brain can be divided and differentiated into the neurons and glia in vitro and this is regulated by a series of growth-factor actions.

Molecular Weight: 178kDa

Gene ID: 10763