alpha Fetoprotein antibody

Catalog No. ABIN722901

Product Details anti-alpha Fetoprotein Antibody

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Rat

Purification: Purified by Protein G.

Immunogen: Full length human AFP protein

Clone: A2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References for anti-alpha Fetoprotein antibody (ABIN722901)

Zhang, Tan, Zhang, Miao, Zou: "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." in: Analytical chemistry, Vol. 88, Issue 13, pp. 6947-53, (2016) (PubMed).

Liu, Zhang, Lu, Li: "3-D microarray and its microfabrication-free fluidic immunoassay device." in: Analytica chimica acta, Vol. 889, pp. 187-93, (2015) (PubMed).

Zhao, Wu, Ju, Yan: "Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition." in: Analytica chimica acta, Vol. 847, pp. 37-43, (2014) (PubMed).

Lin, Mei, Liu, Jin, Wang, Wang: "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." in: Biosensors & bioelectronics, Vol. 66, pp. 177-83, (2014) (PubMed).

Zong, Wu, Xu, Ju, Yan: "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." in: Biosensors & bioelectronics, Vol. 43, pp. 372-8, (2013) (PubMed).

Zong, Wu, Wang, Ju, Yan: "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." in: Analytical chemistry, Vol. 84, Issue 5, pp. 2410-5, (2012) (PubMed).

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP (AFP Products)

Synonyms: FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody

Background:

Synonyms: AFPD, FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP

Background: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process