Recombinant Integrin alpha 5 beta 1 antibody
Reactivity: Human
FACS, IP, Func, IF
Host: Rabbit
Chimeric
SG-19
unconjugated
Recombinant Antibody
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- Target
- Integrin alpha 5 beta 1
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Chimeric
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Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunofluorescence (IF)
- Purpose
- Anti-Integrin alpha 5 beta 1 [SG/19], Rabbit IgG, kappa
- Specificity
- This antibody binds human integrin alpha 5 beta 1 near the N-terminus. Epitope mapping of SG/19 revealed that Thr82 in the 1 subunit, located at the outer face of the boundary between the I-like and hybrid domains, was the key binding determinant for this antibody. Integrin alpha5 beta 1 is also known as fibronectin receptor and binds matrix macromolecules and proteinases and thereby stimulates angiogenesis. Integrin α5β1, is a member of the integrin family, is a heterodimer composed by two subunits, α5 (ITGA5/CD49e) and β1 (ITGB1/CD29), and both are necessary for complete biological functions. The interaction of VLA-5 with fibronectin plays an important role in regulating inflammatory cytokine production by human articular chondrocytes (From the Cell Migration Gateway ITGA5 ITGB1
- Characteristics
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
- Purification
- Protein A affinity purified
- Immunogen
- The original antibody was generated by immunizing BALB/c mice with Nalm-6 cells.
- Clone
- SG-19
- Isotype
- IgG kappa
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- Application Notes
- This antibody was capable of recognizing integrin alpha 5 beta 1 expressed on the surface of K562 cells via immunofluorescence staining. This antibody also precipitated molecules with weight of 110 and 140 kDa under nonreducing conditions from K562 cells and Ramos cells, and 130 kDa when reduced. These results were similar to the ones reported for integrin alpha 5 beta 1. The effect of this antibody on migration beneath stromal cells was investigated by inoculating Nalm-6 and BMS2 cells together with this antibody. It was seen that the presence of this antibody blocked the migration of Nalm-6 cells beneath stromal cells (PMID: 1400595).
- Comment
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- Integrin alpha 5 beta 1
- Background
- CD29, ITGB1, FNRB, MDF2, MSK12, ITGA5:ITGB1, Integrin alpha V beta1, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta
- UniProt
- P05556
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