Recombinant Calponin antibody

Catalog No. ABIN7543405

Product Details anti-Calponin Antibody

Target: Calponin

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Calponin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Calponin protein [24H5], Rabbit IgG, kappa

Specificity: The antibody is specific for calponin protein. Calponin protein is a thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. Further, the interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with Calponin protein.

Clone: 24H5

Isotype: IgG kappa

Application Details

Application Notes: The antibody specifically detected Calponin beta protein in Western blot analysis. The antibody 24H5 and the commercial antibody CALP were used to stain uterine fibroids and liver tissue. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 24H5 was higher than the commercial antibody (CN113234155A).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Calponin

Target: Calponin

Alternative Name: Calponin protein (Calponin Products)

Background: CAP, Calponin-2, Calponin H2, smooth muscle, Neutral calponin, CGMCNO 20775

UniProt: Q99439